ArticleGenetics2004
Conditional expression in the malaria mosquito Anopheles stephensi with Tet-On and Tet-Off systems.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- Engineering Protein Stability with Small Molecules: A Review of the ecDHFR Destabilizing Domain System.ACS chemical biology · 2026Review
- Article
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- Successful insertion and expression of a tetracycline transactivator in Anopheles stephensi associated with increased egg production and decreased hatching rate.Parasites & vectors · 2025Article
- Methylmercury demethylation and volatilization by animals expressing microbial enzymes.Nature communications · 2025Article
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Diverting organic waste from landfills via insect biomanufacturing using engineered black soldier flies (Hermetia illucens).Communications biology · 2024Review
- Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.Scientific reports · 2022Article
- Partial masculinization of Aedes aegypti females by conditional expression of Nix.PLoS neglected tropical diseases · 2022Article
- A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti.Genetics · 2021Article
- A molecular toolkit for superorganisms.Trends in genetics : TIG · 2021Review
- A synthetic male-specific sterilization system using the mammalian pro-apoptotic factor in a malaria vector mosquito.Scientific reports · 2019Article
- A perspective on the need and current status of efficient sex separation methods for mosquito genetic control.Parasites & vectors · 2018Review
- Genomic mining of prokaryotic repressors for orthogonal logic gates.Nature chemical biology · 2014Article
- Article
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Antimicrobial properties of Anopheles albimanus pericardial cells.Cell and tissue research · 2013Article
- Development of the bi-partite Gal4-UAS system in the African malaria mosquito, Anopheles gambiae.PloS one · 2012Article
- Low- and high-tech approaches to control Plasmodium parasite transmission by anopheles mosquitoes.Journal of tropical medicine · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
We report successful conditional gene expression in the malaria vector, Anopheles stephensi, on the basis of binary systems consisting of gene driver and responder transgenic lines generated by Minos-mediated germline transformation. An A. gambiae tissue-specific enhancer derived from a serpin (SRPN10) gene was utilized to control the temporal and spatial expression of doxycycline (dox)-sensitive transcriptional regulators in the driver lines. The "Tet-Off" driver utilized the tetracycline-controlled transcriptional activator (tTA) that is unable to bind and activate transcription from tetracycline operators (TetO) in the presence of dox; the "Tet-on" driver utilized the reverse tTA (rtTA) that, conversely, binds and activates TetO operators in the presence of dox. The responder lines carried insertions encompassing a LacZ reporter gene, cis-regulated by a TetO-P-element hybrid promoter. The progeny of crosses between driver and responder lines expressed beta-galactosidase under dual, tissue-specific and dox-mediated regulation. In adult rtTA/TetOPlacZ progeny, dox treatment rapidly induced beta-galactosidase activity throughout the midgut epithelium and especially in malaria parasite-invaded epithelial cells. Transactivator-dependent, dox-mediated regulation was observed in hemocytes and pericardial cells using both systems. Conditional tissue-specific regulation is a powerful tool for analyzing gene function in mosquitoes and potentially for development of strategies to control disease transmission.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.