ArticleProtein science : a publication of the Protein Society2004
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
Article in Protein science : a publication of the Protein Society, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanism and Control of Meiotic DNA Double-Strand Break Formation inFrontiers in cell and developmental biology · 2021Review
- Structural and functional characterization of the Spo11 core complex.Nature structural & molecular biology · 2021Article
- Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Ski2-like RNA helicase structures: common themes and complex assemblies.RNA biology · 2013Review
- Identifying the hotspots on the top faces of WD40-repeat proteins from their primary sequences by β-bulges and DHSW tetrads.PloS one · 2012Article
- Functional interactions among members of the meiotic initiation complex in fission yeast.Current genetics · 2010Article
- Tethering recombination initiation proteins in Saccharomyces cerevisiae promotes double strand break formation.Genetics · 2009Article
- Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis.Genome dynamics and stability · 2008Article
- The yeast Ski complex is a hetero-tetramer.Protein science : a publication of the Protein Society · 2008Article
- Partner proteins that interact with Clonorchis sinensis WD40-repeat protein.Parasitology research · 2007Article
- Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body.RNA (New York, N.Y.) · 2005Article
- Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.RNA (New York, N.Y.) · 2005Article
- Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome.RNA (New York, N.Y.) · 2005Article
- Under the Tucson sun: a meeting in the desert on mRNA decay.RNA (New York, N.Y.) · 2004Article
- Degradation of mRNAs that lack a stop codon: a decade of nonstop progress.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.
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