ArticleMolecular and cellular biology2004
Essential motifs in the 3' untranslated region required for retrotransposition and the precise start of reverse transcription in non-long-terminal-repeat retrotransposon SART1.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Distinct and overlapping RNA determinants for binding and target-primed reverse transcription by Bombyx mori R2 retrotransposon protein.Nucleic acids research · 2024Article
- Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.Science (New York, N.Y.) · 2023Article
- Telomeric retrotransposons show propensity to form G-quadruplexes in various eukaryotic species.Mobile DNA · 2023Article
- Lessons Learned and Yet-to-Be Learned on the Importance of RNA Structure in SARS-CoV-2 Replication.Microbiology and molecular biology reviews : MMBR · 2022Review
- Recognition of 3'-end L1, Alu, processed pseudogenes, and mRNA stem-loops in the human genome using sequence-based and structure-based machine-learning models.Scientific reports · 2019Article
- Conserved 3' UTR stem-loop structure in L1 and Alu transposons in human genome: possible role in retrotransposition.BMC genomics · 2016Article
- Both the Exact Target Site Sequence and a Long Poly(A) Tail Are Required for Precise Insertion of the 18S Ribosomal DNA-Specific Non-Long Terminal Repeat Retrotransposon R7Ag.Molecular and cellular biology · 2016Article
- Insertion of Retrotransposons at Chromosome Ends: Adaptive Response to Chromosome Maintenance.Frontiers in genetics · 2015Review
- Mechanism by which a LINE protein recognizes its 3' tail RNA.Nucleic acids research · 2014Article
- The specificity and flexibility of l1 reverse transcription priming at imperfect T-tracts.PLoS genetics · 2013Article
- RNA-Mediated Gene Duplication and Retroposons: Retrogenes, LINEs, SINEs, and Sequence Specificity.International journal of evolutionary biology · 2013Article
- Parallel relaxation of stringent RNA recognition in plant and mammalian L1 retrotransposons.Molecular biology and evolution · 2012Article
- Retrotransposons at Drosophila telomeres: host domestication of a selfish element for the maintenance of genome integrity.Biochimica et biophysica acta · 2012Review
- Non-long terminal repeat (non-LTR) retrotransposons: mechanisms, recent developments, and unanswered questions.Mobile DNA · 2010Article
- Essential domains for ribonucleoprotein complex formation required for retrotransposition of telomere-specific non-long terminal repeat retrotransposon SART1.Molecular and cellular biology · 2006Article
- Solution structure and functional importance of a conserved RNA hairpin of eel LINE UnaL2.Nucleic acids research · 2006Article
- Eukaryotic translational coupling in UAAUG stop-start codons for the bicistronic RNA translation of the non-long terminal repeat retrotransposon SART1.Molecular and cellular biology · 2005Article
- Telomere-specific non-LTR retrotransposons and telomere maintenance in the silkworm, Bombyx mori.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- Functional roles of 3'-terminal structures of template RNA during in vivo retrotransposition of non-LTR retrotransposon, R1Bm.Nucleic acids research · 2005Article
- The Wide Distribution and Change of Target Specificity of R2 Non-LTR Retrotransposons in Animals.PloS oneArticle
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-long-terminal-repeat (non-LTR) retrotransposons amplify their copies by reverse transcribing mRNA from the 3' end, but the initial processes of reverse transcription are still unclear. We have shown that a telomere-specific non-LTR retrotransposon of the silkworm, SART1, requires the 3' untranslated region (3' UTR) for retrotransposition. With an in vivo retrotransposition assay, we identified several novel motifs within the 3' UTR involved in precise and efficient reverse transcription. Of 461 nucleotides (nt) of the 3' UTR, the central region, from nt 163 to nt 295, was essential for SART1 retrotransposition. Of five putative stem-loops formed in RNA for the SART1 3' UTR, the second stem-loop (nt 159 to 221) is included in this region. Loss of the 3' region (nt 296 to 461) in the 3' UTR and the poly(A) tract resulted in decreased and inaccurate reverse transcription, which starts mostly from several telomeric repeat-like GGUU sequences just downstream of the second stem-loop. These results suggest that short telomeric repeat-like sequences in the 3' UTR anneal to the bottom strand of (TTAGG)(n) repeats. We also demonstrated that the mRNA for green fluorescent protein (GFP) could be retrotransposed into telomeric repeats when the GFP coding region is fused with the SART1 3' UTR and SART1 open reading frame proteins are supplied in trans.
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