ArticleMolecular and cellular biology2004
Nature of the accessible chromatin at a glucocorticoid-responsive enhancer.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
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- A Microfluidic Device with Integrated Sonication and Immunoprecipitation for Sensitive Epigenetic Assays.Analytical chemistry · 2016Article
- Sirt1 ablation promotes stress-induced loss of epigenetic and genomic hematopoietic stem and progenitor cell maintenance.The Journal of experimental medicine · 2013Article
- Control of nuclear receptor function by local chromatin structure.The FEBS journal · 2011Review
- DNA methylation status predicts cell type-specific enhancer activity.The EMBO journal · 2011Article
- Multiple distinct stimuli increase measured nucleosome occupancy around human promoters.PloS one · 2011Article
- Human T-lymphotropic virus type 1 transcription and chromatin-remodeling complexes.Journal of virology · 2010Article
- Highly compacted chromatin formed in vitro reflects the dynamics of transcription activation in vivo.Molecular cell · 2010Article
- Hypoxic repression of endothelial nitric-oxide synthase transcription is coupled with eviction of promoter histones.The Journal of biological chemistry · 2010Article
- Multiple sequence-directed possibilities provide a pool of nucleosome position choices in different states of activity of a gene.Epigenetics & chromatin · 2009Article
- Molecular basis of CD4 repression by the Swi/Snf-like BAF chromatin remodeling complex.European journal of immunology · 2009Article
- Histone modifications, but not nucleosomal positioning, correlate with major histocompatibility complex class I promoter activity in different tissues in vivo.Molecular and cellular biology · 2008Article
- Active cytosine demethylation triggered by a nuclear receptor involves DNA strand breaks.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Targeting chromosomal sites with locked nucleic acid-modified triplex-forming oligonucleotides: study of efficiency dependence on DNA nuclear environment.Nucleic acids research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To gain a better understanding of the nature of active chromatin in mammals, we have characterized in living cells the various chromatin modification events triggered by the glucocorticoid receptor (GR) at the rat tyrosine aminotransferase gene. GR promotes a local remodeling at a glucocorticoid-responsive unit (GRU) located 2.5 kb upstream of the transcription start site, creating nuclease hypersensitivity that encompasses 450 bp of DNA. Nucleosomes at the GRU occupy multiple frames that are remodeled without nucleosome repositioning, showing that nucleosome positioning is not the key determinant of chromatin accessibility at this locus. Remodeling affects nucleosomes and adjacent linker sequences, enhancing accessibility at both regions. This is associated with decreased interaction of both the linker histone H1 and the core histone H3 with DNA. Thus, our results indicate that nucleosome and linker histone removal rather than nucleosome repositioning is associated with GR-triggered accessibility. Interestingly, GR induces hyperacetylation of histones H3 and H4, but this is not sufficient either for remodeling or for transcriptional activation. Finally, our data favor the coexistence of several chromatin states within the population, which may account for the previously encountered difficulties in characterizing unambiguously the active chromatin structure in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.