ArticleBiophysical journal2004
Structure and transport mechanism of the bacterial oxalate transporter OxlT.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Adaptively evolvedApplied and environmental microbiology · 2025Article
- A membrane protein of the rice pathogen Burkholderia glumae required for oxalic acid secretion and quorum sensing.Molecular plant pathology · 2023Article
- Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota.Nature communications · 2023Article
- The Genetic Architecture of Emerging Fungicide Resistance in Populations of a Global Wheat Pathogen.Genome biology and evolution · 2020Article
- Applications of sequence coevolution in membrane protein biochemistry.Biochimica et biophysica acta. Biomembranes · 2018Review
- Diversity and ecology of oxalotrophic bacteria.World journal of microbiology & biotechnology · 2016Review
- Homology modeling, molecular dynamics, and virtual screening of NorA efflux pump inhibitors ofDrug design, development and therapy · 2016Article
- Flexible gates generate occluded intermediates in the transport cycle of LacY.Journal of molecular biology · 2014Article
- Protonation drives the conformational switch in the multidrug transporter LmrP.Nature chemical biology · 2014Article
- Structural requirements for the activity of the MirB ferrisiderophore transporter of Aspergillus fumigatus.Eukaryotic cell · 2012Article
- Mutational analysis of the respiratory nitrate transporter NarK2 of Mycobacterium tuberculosis.PloS one · 2012Article
- Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation.BMC genomics · 2011Article
- Capturing Functional Motions of Membrane Channels and Transporters with Molecular Dynamics Simulation.Journal of computational and theoretical nanoscience · 2010Article
- Structural perspectives on secondary active transporters.Trends in pharmacological sciences · 2010Review
- Conformational Propensities of Peptides Mimicking Transmembrane Helix 5 and Motif C in Wild-type and Mutant Vesicular Acetylcholine Transporters.ACS chemical neuroscience · 2010Article
- Structural basis of substrate selectivity in the glycerol-3-phosphate: phosphate antiporter GlpT.Biophysical journal · 2009Article
- Vitamin C transporters.Journal of physiology and biochemistry · 2008Review
- Ins and outs of major facilitator superfamily antiporters.Annual review of microbiology · 2008Review
- Changes in secondary structures and acidic side chains of melibiose permease upon cosubstrates binding.Biophysical journal · 2006Article
- Experimental tests of a homology model for OxlT, the oxalate transporter of Oxalobacter formigenes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Membrane proteins that belong to the major facilitator superfamily (MFS) are found in organisms across the evolutionary spectrum and mediate the transport of a variety of substrates ranging from small metabolites to neurotransmitters. The oxalate transporter (OxlT) is a representative MFS protein, and exchanges formate for oxalate across the cytoplasmic membrane of the organism Oxalobacter formigenes. Here, we present a structural model for the protein conformational changes that occur during oxalate transport by combining a three-dimensional map of the oxalate-bound, "closed" state of OxlT at 6.5 A determined by cryo-electron microscopy with a model of the "open" state of OxlT based on the atomic structures of the related transporters, glycerol-3-phosphate transporter (GlpT) and lactose permease (LacY). We demonstrate that the principal structural change associated with substrate transport is a concerted rocking movement of the two structurally similar halves of the protein relative to each other. Our structural model places two positively charged residues, Arg-272 and Lys-355 in the central cavity, suggesting that electrostatic interactions between these residues and the oxalate anion is a key step in generating the conformational change between the open and closed states of the transporter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.