ArticleProceedings of the National Academy of Sciences of the United States of America1992
Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- KnotResolver: tracking self-intersecting filaments in microscopy using directed graphs.Bioinformatics (Oxford, England) · 2024Article
- Purification of Human Cytoplasmic Actins FromBio-protocol · 2023Article
- Enterovirus pathogenesis requires the host methyltransferase SETD3.Nature microbiology · 2019Article
- The extreme C terminus of theThe Journal of biological chemistry · 2018Article
- Roles of Asp179 and Glu270 in ADP-Ribosylation of Actin by Clostridium perfringens Iota Toxin.PloS one · 2015Article
- Biochemical and bioinformatic analysis of the myosin-XIX motor domain.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Cofilin-linked changes in actin filament flexibility promote severing.Biophysical journal · 2011Article
- The Princeton Protein Orthology Database (P-POD): a comparative genomics analysis tool for biologists.PloS one · 2007Article
- A genetic dissection of Aip1p's interactions leads to a model for Aip1p-cofilin cooperative activities.Molecular biology of the cell · 2006Article
- Differential interaction of cardiac, skeletal muscle, and yeast tropomyosins with fluorescent (pyrene235) yeast actin.Biophysical journal · 2006Article
- Glycolytic enzyme interactions with yeast and skeletal muscle F-actin.Biophysical journal · 2006Article
- Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p.The Journal of cell biology · 1998Article
- Rapid and efficient purification of actin from nonmuscle sources.Cell motility and the cytoskeleton · 1998Article
- High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.The Journal of cell biology · 1997Article
- Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.The Journal of cell biology · 1996Article
- Actin filaments in yeast are unstable in the absence of capping protein or fimbrin.The Journal of cell biology · 1995Article
- Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Actomyosin kinetics and in vitro motility of wild-type Drosophila actin and the effects of two mutations in the Act88F gene.Biophysical journal · 1995Article
- What are the basic functions of microfilaments? Insights from studies in budding yeast.The Journal of cell biology · 1994Review
- Mapping actin surfaces required for functional interactions in vivo.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Saccharomyces cerevisiae has been used to study the function of components of the actin cytoskeleton in vivo, mainly because it is easy to derive and characterize mutations affecting these proteins. In contrast, biochemical studies have generally used proteins derived from higher eukaryotes. We have devised a simple procedure to prepare, in high yield, homogeneous native actin from wild-type and act1 mutant yeast. Using intensified video fluorescence microscopy, we found that actin filaments polymerized from these preparations exhibit ATP-dependent sliding movement over surfaces coated with rabbit skeletal muscle myosin. The rates of sliding movement of the wild-type and mutant yeast actins were each about half that of rabbit skeletal muscle actin under similar conditions. We conclude that over the large evolutionary distance between yeast and mammals there has been significant conservation of actin function, specifically the ability to be moved by interaction with myosin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.