ArticleThe Biochemical journal2004
Cytochrome c oxidase subunit IV as a marker of protein kinase Cepsilon function in neonatal cardiac myocytes: implications for cytochrome c oxidase activity.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- CytochromeCells · 2021Review
- The peri-menopause in a woman's life: a systemic inflammatory phase that enables later neurodegenerative disease.Journal of neuroinflammation · 2020Review
- PKC and PKN in heart disease.Journal of molecular and cellular cardiology · 2019Review
- Protein kinase Cε targets respiratory chain and mitochondrial membrane potential but not FJournal of cellular biochemistry · 2018Article
- Ischemia/Reperfusion-Induced Translocation of PKCβII to Mitochondria as an Important Mediator of a Protective Signaling Mechanism in an Ischemia-Resistant Region of the Hippocampus.Neurochemical research · 2017Article
- Revisiting Kadenbach: Electron flux rate through cytochrome c-oxidase determines the ATP-inhibitory effect and subsequent production of ROS.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Energy crisis: the role of oxidative phosphorylation in acute inflammation and sepsis.Biochimica et biophysica acta · 2014Review
- Oral contraceptives and nicotine synergistically exacerbate cerebral ischemic injury in the female brain.Translational stroke research · 2013Article
- Selective activation of protein kinase C∊ in mitochondria is neuroprotective in vitro and reduces focal ischemic brain injury in mice.Journal of neuroscience research · 2013Article
- Article
- Article
- Cardiac mitochondrial matrix and respiratory complex protein phosphorylation.American journal of physiology. Heart and circulatory physiology · 2012Review
- Regulation of mitochondrial respiration and apoptosis through cell signaling: cytochrome c oxidase and cytochrome c in ischemia/reperfusion injury and inflammation.Biochimica et biophysica acta · 2012Review
- Article
- Article
- Phosphorylation of mammalian cytochrome c and cytochrome c oxidase in the regulation of cell destiny: respiration, apoptosis, and human disease.Advances in experimental medicine and biology · 2012Review
- Protein kinase C-epsilon activation induces mitochondrial dysfunction and fragmentation in renal proximal tubules.American journal of physiology. Renal physiology · 2011Article
- What can we learn about cardioprotection from the cardiac mitochondrial proteome?Cardiovascular research · 2010Review
- Reactive oxygen species originating from mitochondria regulate the cardiac sodium channel.Circulation research · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously demonstrated that low concentrations of phorbol esters stimulate the selective translocation of protein kinase C (PKC) alpha and epsilon from the cell soluble to the particulate fraction in NCMs (neonatal rat cardiac myocytes). We therefore determined if the in vitro phosphorylation of substrates in these fractions could be used as assays of PKCalpha or epsilon activation. Intact cell phorbol ester treatment caused a decline in the in vitro (32)P-incorporation into several proteins in the cell-soluble fraction. These declines occurred in the presence or absence of in vitro Ca(2+) and probably reflected the exit of PKC isoenzymes from the soluble fraction. In contrast, an approx. 18 kDa protein incorporated (32)P in particulate fractions isolated from 4beta-PMA-treated cells in a Ca(2+)-independent manner. Proteomic and immunoprecipitation analyses indicated that the protein is subunit IV of the cytochrome c oxidase complex (COIV). In vitro phosphorylation of COIV was attenuated by PKC pseudosubstrate peptides. Introduction of an PKCepsilon-selective translocation inhibitor [Johnson, Gray, Chen and Mochly-Rosen (1996) J. Biol. Chem. 271, 24962-24966] into NCMs before 4beta-PMA treatments also attenuated the in vitro phosphorylation of COIV. In mitochondrial extracts from 4beta-PMA-treated NCMs, the PKCepsilon isoenzyme coimmunoprecipitated with COIV, and cytochrome c oxidase activity was enhanced 2-fold. The in vitro phosphorylation of COIV reflects a novel approach for monitoring PKCepsilon function in NCMs. Furthermore, PKCepsilon probably interacts with COIV in NCM mitochondria to enhance electron-transport chain complex IV activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.