ArticlePhotochemistry and photobiology
Spatial control of reactive oxygen species formation in fibroblasts using two-photon excitation.
Article in Photochemistry and photobiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Integrated Topical Skincare Enhances Picosecond Laser Treatment Outcomes for Photoaging: A Prospective Split-Face Trial.Aesthetic plastic surgery · 2026Trial
- Vitamin D-loaded lipid nanoparticles: antioxidant properties, preparation, optimization, and in vitro characterization.Drug delivery and translational research · 2025Article
- Impact of polyethylene terephthalate nanoplastics (PET) on fibroblasts: a study on NIH-3T3 cells.Frontiers in physiology · 2025Article
- Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages.International journal of environmental research and public health · 2022Article
- The Effect of Quasi-Spherical Gold Nanoparticles on Two-Photon Induced Reactive Oxygen Species for Cell Damage.Nanomaterials (Basel, Switzerland) · 2021Article
- Experimental models and methods for cutaneous wound healing assessment.International journal of experimental pathology · 2020Review
- Epigallocatechin-3-gallate induces oxidative phosphorylation by activating cytochrome c oxidase in human cultured neurons and astrocytes.Oncotarget · 2016Article
- Voriconazole N-oxide and its ultraviolet B photoproduct sensitize keratinocytes to ultraviolet A.The British journal of dermatology · 2015Article
- Mitigating phototoxicity during multiphoton microscopy of live Drosophila embryos in the 1.0-1.2 µm wavelength range.PloS one · 2014Article
- High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation.Scientific reports · 2013Article
- Kinetics of endogenous mouse FEN1 in base excision repair.Nucleic acids research · 2012Article
- The activity-induced reduction of myofibrillar Ca2+ sensitivity in mouse skeletal muscle is reversed by dithiothreitol.The Journal of physiology · 2006Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Two-photon excitation (2PE) provides a means of generating reactive oxygen species (ROS) in cells and tissues with a high degree of spatial specificity. In cultured monolayers of human fibroblasts and fibroblast-derived cells treated with the commonly used probe of ROS formation, 5-(and 6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H(2)DCFDA), cells irradiated through a microscope objective with 150 fs near-infrared laser pulses became highly fluorescent, reflecting intracellular ROS formation. The fluorescence intensity inside cells increased quadratically with the average power of radiation for pulsed excitation and was unchanged for continuous wave irradiation with the same average power. Single fibroblasts embedded within dermal equivalents were also targeted in this manner and formed ROS, whereas neighboring unirradiated cells were spared. These results demonstrate that ROS can be generated intracellularly in skin cells using 2PE of the metabolic or oxidative products of CM-H(2)DCFDA and that formation of ROS can be localized in both cell monolayers and in a tissue equivalent. This technique should be useful in understanding the response of whole tissues such as skin to local generation of ROS and may have applications in photodynamic therapy.
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Registered trials
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