ArticlePlant cell reports2004
Agrobacterium-mediated transformation of European chestnut embryogenic cultures.
Article in Plant cell reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Overexpression of ginkbilobin-2 homologous domain gene to enhance the tolerance to Phytophthora cinnamomi in plants of European chestnut.BMC genomics · 2026Article
- First Report on Genome Editing via Ribonucleoprotein (RNP) inInternational journal of molecular sciences · 2022Article
- Article
- European and American chestnuts: An overview of the main threats and control efforts.Frontiers in plant science · 2022Review
- First Report of CRISPR/Cas9 Gene Editing inFrontiers in plant science · 2021Article
- Stable Agrobacterium-mediated transformation of maritime pine based on kanamycin selection.TheScientificWorldJournal · 2013Article
- Agrobacterium rhizogenes-mediated transformation of Prunus as an alternative for gene functional analysis in hairy-roots and composite plants.Plant cell reports · 2011Article
- Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur.Plant cell reports · 2010Article
- Sexually mature transgenic American chestnut trees via embryogenic suspension-based transformation.Plant cell reports · 2009Article
- Development of an Agrobacterium-mediated transformation protocol for the tree-legume Leucaena leucocephala using immature zygotic embryos.Plant cell, tissue and organ culture · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An innovative and efficient genetic transformation protocol for European chestnut is described in which embryogenic cultures are used as the target material. When somatic embryos at the globular or early-torpedo stages were cocultured for 4 days with Agrobacterium tumefaciens strain EHA105 harbouring the pUbiGUSINT plasmid containing marker genes, a transformation efficiency of 25% was recorded. Murashige and Skoog culture medium containing 150 mg/l of kanamycin was used as the selection medium. The addition of acetosyringone was detrimental to the transformation efficiency. Transformation was confirmed by a histochemical beta-glucuronidase (GUS ) assay, PCR and Southern blot analyses for the uidA (GUS) and nptII (neomycin phosphotransferase II) genes. At present, 93 GUS-positive chestnut embryogenic lines are being maintained in culture. Low germination rates (6.3%) were recorded for the transformed somatic embryos. The presence of the transferred genes in leaves and shoots derived from the germinated embryos was also verified by the GUS assay and PCR analysis.
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15338188What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.