ArticleRNA (New York, N.Y.)2004
Intracellular folding of the Tetrahymena group I intron depends on exon sequence and promoter choice.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Moderate activity of RNA chaperone maximizes the yield of self-spliced pre-RNA in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Topological crossing in the misfoldedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A roadmap for rRNA folding and assembly during transcription.Trends in biochemical sciences · 2021Review
- Mimicking Co-Transcriptional RNA Folding Using a Superhelicase.Journal of the American Chemical Society · 2018Article
- Transcription elongation rate affects nascent histone pre-mRNA folding and 3' end processing.Genes & development · 2018Article
- Detecting riboSNitches with RNA folding algorithms: a genome-wide benchmark.Nucleic acids research · 2015Article
- Review
- Molecular crowding overcomes the destabilizing effects of mutations in a bacterial ribozyme.Nucleic acids research · 2015Article
- Exploiting post-transcriptional regulation to probe RNA structures in vivo via fluorescence.Nucleic acids research · 2015Article
- Trans-splicing with the group I intron ribozyme from Azoarcus.RNA (New York, N.Y.) · 2014Article
- On the importance of cotranscriptional RNA structure formation.RNA (New York, N.Y.) · 2013Review
- Transient RNA structure features are evolutionarily conserved and can be computationally predicted.Nucleic acids research · 2013Article
- mRNA secondary structures fold sequentially but exchange rapidly in vivo.PLoS biology · 2010Article
- Communication between RNA folding domains revealed by folding of circularly permuted ribozymes.Journal of molecular biology · 2007Article
- Distinct contribution of electrostatics, initial conformational ensemble, and macromolecular stability in RNA folding.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Self-splicing of a group I intron reveals partitioning of native and misfolded RNA populations in yeast.RNA (New York, N.Y.) · 2006Article
- The role of a metastable RNA secondary structure in hepatitis delta virus genotype III RNA editing.RNA (New York, N.Y.) · 2006Article
- RNA folding in living cells.RNA biologyReview
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Tetrahymena group I intron splices 20 to 50 times faster in Tetrahymena than in vitro, implying that the intron rapidly adopts its active conformation in the cell. The importance of cotranscriptional folding and the contribution of the rRNA exons to the stability of the active pre-RNA structure were investigated by comparing the activity of minimal pre-RNAs expressed in Escherichia coli. Pre-RNAs containing exons derived from E. coli 23 S rRNA were three to four times more active than the wild-type Tetrahymena pre-RNA. E. coli transcripts of the chimeric E. coli pre-RNA were two to eight times more active than were T7 transcripts. However, the effect of cotranscriptional folding depends on exon sequences. Unexpectedly, the unspliced pre-RNA decays more slowly than predicted from the rate of splicing. This observation is best explained by partitioning of transcripts into active and inactive pools. We propose that the active pool splices within a few seconds, whereas the inactive pool is degraded without appreciable splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.