ArticleNucleic acids research2004
Quantitative assessment of a novel flow-through porous microarray for the rapid analysis of gene expression profiles.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Lab-in-a-Tip: a multiplex immunoassay platform based on a self-assembled barcoded protein array.Nature communications · 2025Article
- Novel EPHB4 Receptor Tyrosine Kinase Mutations and Kinomic Pathway Analysis in Lung Cancer.Scientific reports · 2015Article
- Development of a flow-through [corrected] microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of European Bunyaviruses. [corrected].Molecular biotechnology · 2011Article
- Rapid screening for chromosomal aneuploidies using array-MLPA.BMC medical genetics · 2011Article
- MOF and histone H4 acetylation at lysine 16 are critical for DNA damage response and double-strand break repair.Molecular and cellular biology · 2010Article
- A preliminary result of three-dimensional microarray technology to gene analysis with endoscopic ultrasound-guided fine-needle aspiration specimens and pancreatic juices.Journal of experimental & clinical cancer research : CR · 2010Article
- The Dienes phenomenon: competition and territoriality in Swarming Proteus mirabilis.Journal of bacteriology · 2009Article
- Channel glass-based detection of human short insertion/deletion polymorphisms by tandem hybridization.Molecular biotechnology · 2008Article
- A comparison of hybridization efficiency between flat glass and channel glass solid supports.Molecular biotechnology · 2008Article
- The micro-Petri dish, a million-well growth chip for the culture and high-throughput screening of microorganisms.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Article
- Microarray MAPH: accurate array-based detection of relative copy number in genomic DNA.BMC genomics · 2006Article
- Growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel microarray system that utilizes a porous aluminum-oxide substrate and flow-through incubation has been developed for rapid molecular biological testing. To assess its utility in gene expression analysis, we determined hybridization kinetics, variability, sensitivity and dynamic range of the system using amplified RNA. To show the feasibility with complex biological RNA, we subjected Jurkat cells to heat-shock treatment and analyzed the transcriptional regulation of 23 genes. We found that trends (regulation or no change) acquired on this platform are in good agreement with data obtained from real-time quantitative PCR and Affymetrix GeneChips. Additionally, the system demonstrates a linear dynamic range of 3 orders of magnitude and at least 10-fold decreased hybridization time compared to conventional microarrays. The minimum amount of transcript that could be detected in 20 microl volume is 2-5 amol, which enables the detection of 1 in 300,000 copies of a transcript in 1 microg of amplified RNA. Hybridization and subsequent analysis are completed within 2 h. Replicate hybridizations on 24 identical arrays with two complex biological samples revealed a mean coefficient of variation of 11.6%. This study shows the potential of flow-through porous microarrays for the rapid analysis of gene expression profiles in clinical applications.
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