ArticleMolecular biology of the cell2004
In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 88 citations in OpenAlex.
- Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles.The Journal of cell biology · 2026Article
- RAB23 facilitates clathrin-coated nascent vesicle formation at the plasma membrane and modulates cell signaling.Cellular and molecular life sciences : CMLS · 2025Article
- A retrotransposon-derived DNA zip code internalizes myeloma cells through Clathrin-Rab5a-mediated endocytosis.Frontiers in oncology · 2024Article
- The Emerging Role of Rab5 in Membrane Receptor Trafficking and Signaling Pathways.Biochemistry research international · 2020Review
- Protein interacting with Amyloid Precursor Protein tail-1 (PAT1) is involved in early endocytosis.Cellular and molecular life sciences : CMLS · 2019Article
- CMTM4 regulates angiogenesis by promoting cell surface recycling of VE-cadherin to endothelial adherens junctions.Angiogenesis · 2019Article
- Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles.Journal of cell science · 2018Article
- VGLUT2 Trafficking Is Differentially Regulated by Adaptor Proteins AP-1 and AP-3.Frontiers in cellular neuroscience · 2017Article
- Article
- Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.The Journal of cell biology · 2016Article
- Rab5 and Rab4 regulate axon elongation in the Xenopus visual system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Rabaptin-5 and Rabex-5 are neoplastic tumour suppressor genes that interact to modulate Rab5 dynamics in Drosophila melanogaster.Developmental biology · 2014Article
- The cell-based L-glutathione protection assays to study endocytosis and recycling of plasma membrane proteins.Journal of visualized experiments : JoVE · 2013Article
- Αvβ3-integrin-mediated adhesion is regulated through an AAK1L- and EHD3-dependent rapid-recycling pathway.Journal of cell science · 2013Article
- Vesicles modulate an actin network for asymmetric spindle positioning.Nature cell biology · 2013Article
- Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway.Journal of cell science · 2013Article
- Adaptor protein complexes 1 and 3 are essential for generation of synaptic vesicles from activity-dependent bulk endosomes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Residues in the hendra virus fusion protein transmembrane domain are critical for endocytic recycling.Journal of virology · 2012Article
- Rabs and EHDs: alternate modes for traffic control.Bioscience reports · 2012Review
- Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The involvement of clathrin and associated adaptor proteins in receptor recycling from endosomes back to the plasma membrane is controversial. We have used an in vitro assay to identify the molecular requirements for the formation of recycling vesicles. Cells expressing the asialoglycoprotein receptor H1, a typical recycling receptor, were surface biotinylated and then allowed to endocytose for 10 min. After stripping away surface-biotin, the cells were permeabilized and the cytosol washed away. In a temperature-, cytosol-, and nucleotide-dependent manner, the formation of sealed vesicles containing biotinylated H1 could be reconstituted. Vesicle formation was strongly inhibited upon immunodepletion of adaptor protein (AP)-1, but not of AP-2 or AP-3, from the cytosol, and was restored by readdition of purified AP-1. Vesicle formation was stimulated by supplemented clathrin, but inhibited by brefeldin A, consistent with the involvement of ARF1 and a brefeldin-sensitive guanine nucleotide exchange factor. The GTPase rab4, but not rab5, was required to generate endosome-derived vesicles. Depletion of rabaptin-5/rabex-5, a known interactor of both rab4 and gamma-adaptin, stimulated and addition of the purified protein strongly inhibited vesicle production. The results indicate that recycling is mediated by AP-1/clathrin-coated vesicles and regulated by rab4 and rabaptin-5/rabex-5.
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