ArticleMolecular biology of the cell2004
Targeted disruption of Drosophila Roc1b reveals functional differences in the Roc subunit of Cullin-dependent E3 ubiquitin ligases.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56.The Journal of cell biology · 2021Article
- CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16Cancer biology & medicine · 2019Article
- Analysis of Drosophila melanogaster testis transcriptome.BMC genomics · 2018Article
- A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning.PLoS biology · 2013Article
- Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.PLoS pathogens · 2012Article
- Defining components of the ß-catenin destruction complex and exploring its regulation and mechanisms of action during development.PloS one · 2012Article
- Induction of cell senescence by targeting to Cullin-RING Ligases (CRLs) for effective cancer therapy.International journal of biochemistry and molecular biology · 2012Article
- SAG/RBX2/ROC2 E3 ubiquitin ligase is essential for vascular and neural development by targeting NF1 for degradation.Developmental cell · 2011Article
- Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype.The Journal of cell biology · 2011Article
- Small RING Finger Proteins RBX1 and RBX2 of SCF E3 Ubiquitin Ligases: The Role in Cancer and as Cancer Targets.Genes & cancer · 2010Article
- RBX1/ROC1-SCF E3 ubiquitin ligase is required for mouse embryogenesis and cancer cell survival.Cell division · 2009Article
- RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
- Evolutionary origin of regulatory regions of retrogenes in Drosophila.BMC genomics · 2008Article
- A ubiquitin ligase complex regulates caspase activation during sperm differentiation in Drosophila.PLoS biology · 2007Article
- Article
- VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.Genes & development · 2004Article
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5 authors.
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Abstract
Cullin-dependent ubiquitin ligases regulate a variety of cellular and developmental processes by recruiting specific proteins for ubiquitin-mediated degradation. Cullin proteins form a scaffold for two functional modules: a catalytic module comprised of a small RING domain protein Roc1/Rbx1 and a ubiquitin-conjugating enzyme (E2), and a substrate recruitment module containing one or more proteins that bind to and bring the substrate in proximity to the catalytic module. Here, we present evidence that the three Drosophila Roc proteins are not functionally equivalent. Mutation of Roc1a causes lethality that cannot be rescued by expression of Roc1b or Roc2 by using the Roc1a promoter. Roc1a mutant cells hyperaccumulate Cubitus interruptus, a transcription factor that mediates Hedgehog signaling. This phenotype is not rescued by expression of Roc2 and only partially by expression of Roc1b. Targeted disruption of Roc1b causes male sterility that is partially rescued by expression of Roc1a by using the Roc1b promoter, but not by similar expression of Roc2. These data indicate that Roc proteins play nonredundant roles during development. Coimmunoprecipitation followed by Western or mass spectrometric analysis indicate that the three Roc proteins preferentially bind certain Cullins, providing a possible explanation for the distinct biological activities of each Drosophila Roc/Rbx.
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