ArticleThe EMBO journal2004
Transcarboxylase 5S structures: assembly and catalytic mechanism of a multienzyme complex subunit.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- Structural Characterization of Multienzyme Assemblies: An Overview.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Carbon Dioxide and the Carbamate Post-Translational Modification.Frontiers in molecular biosciences · 2022Review
- The identification of carbon dioxide mediated protein post-translational modifications.Nature communications · 2018Article
- Analyses of cobalt-ligand and potassium-ligand bond lengths in metalloproteins: trends and patterns.Journal of molecular modeling · 2014Article
- Article
- Insights into the carboxyltransferase reaction of pyruvate carboxylase from the structures of bound product and intermediate analogs.Biochemical and biophysical research communications · 2013Article
- Crystal structures of vertebrate dihydropyrimidinase and complexes from Tetraodon nigroviridis with lysine carbamylation: metal and structural requirements for post-translational modification and function.The Journal of biological chemistry · 2013Article
- Structure and function of biotin-dependent carboxylases.Cellular and molecular life sciences : CMLS · 2013Review
- The enzymes of biotin dependent CO₂ metabolism: what structures reveal about their reaction mechanisms.Protein science : a publication of the Protein Society · 2012Review
- The complete genome of Propionibacterium freudenreichii CIRM-BIA1, a hardy actinobacterium with food and probiotic applications.PloS one · 2010Article
- Article
- Probing the catalytic roles of Arg548 and Gln552 in the carboxyl transferase domain of the Rhizobium etli pyruvate carboxylase by site-directed mutagenesis.Biochemistry · 2010Article
- A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.Structure (London, England : 1993) · 2009Article
- Insight into the carboxyl transferase domain mechanism of pyruvate carboxylase from Rhizobium etli.Biochemistry · 2009Article
- Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism.The Journal of biological chemistry · 2009Article
- Stimulation of mammalian G-protein-responsive adenylyl cyclases by carbon dioxide.The Journal of biological chemistry · 2009Article
- Structure, mechanism and regulation of pyruvate carboxylase.The Biochemical journal · 2008Review
- Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase.Journal of bacteriology · 2007Article
- The crystal structure of Escherichia coli TdcF, a member of the highly conserved YjgF/YER057c/UK114 family.BMC structural biology · 2007Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Transcarboxylase is a 1.2 million Dalton (Da) multienzyme complex from Propionibacterium shermanii that couples two carboxylation reactions, transferring CO(2)(-) from methylmalonyl-CoA to pyruvate to yield propionyl-CoA and oxaloacetate. Crystal structures of the 5S metalloenzyme subunit, which catalyzes the second carboxylation reaction, have been solved in free form and bound to its substrate pyruvate, product oxaloacetate, or inhibitor 2-ketobutyrate. The structure reveals a dimer of beta(8)alpha(8) barrels with an active site cobalt ion coordinated by a carbamylated lysine, except in the oxaloacetate complex in which the product's carboxylate group serves as a ligand instead. 5S and human pyruvate carboxylase (PC), an enzyme crucial to gluconeogenesis, catalyze similar reactions. A 5S-based homology model of the PC carboxyltransferase domain indicates a conserved mechanism and explains the molecular basis of mutations in lactic acidemia. PC disease mutations reproduced in 5S result in a similar decrease in carboxyltransferase activity and crystal structures with altered active sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.