ArticleThe Journal of cell biology2004
Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 1 of them a synthesis that pooled it.
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Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 194 citations in OpenAlex.
- Exosome Biogenesis: Meta-Analysis of Intraluminal Vesicle Size Across Species.International journal of molecular sciences · 2026Pooled it
- ESCRT-III function in membrane fission and repair.Nature reviews. Molecular cell biology · 2026Review
- Host ESCRT machinery orchestrates the assembly of tomato spotted wilt virus ribonucleoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- 80 years of extracellular vesicles: from discovery to clinical translation.Extracellular vesicles and circulating nucleic acids · 2026Review
- Wbm0152, an outer membrane lipoprotein of the Wolbachia endosymbiont of Brugia malayi, inhibits yeast ESCRT complex activity.PLoS pathogens · 2025Article
- The Machinery of Exosomes: Biogenesis, Release, and Uptake.International journal of molecular sciences · 2023Review
- The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis.Frontiers in plant science · 2023Article
- Role of Ceramides and Lysosomes in Extracellular Vesicle Biogenesis, Cargo Sorting and Release.International journal of molecular sciences · 2022Review
- Bro1 family proteins harmonize cargo sorting with vesicle formation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4.Traffic (Copenhagen, Denmark) · 2022Article
- Membrane Trafficking Proteins: A New Target to Identify Resistance to Viruses in Plants.Plants (Basel, Switzerland) · 2021Review
- Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis.The Journal of cell biology · 2021Article
- Vacuolar H+-ATPase dysfunction rescues intralumenal vesicle cargo sorting in yeast lacking PI(3,5)P2 or Doa4.Journal of cell science · 2021Article
- Mitochondria-Associated Degradation Pathway (MAD) Function beyond the Outer Membrane.Cell reports · 2020Article
- The putative deubiquitinating enzyme MoUbp4 is required for infection-related morphogenesis and pathogenicity in the rice blast fungus Magnaporthe oryzae.Current genetics · 2020Article
- Identification and characterization of a protein Bro1 essential for sophorolipids synthesis in Starmerella bombicola.Journal of industrial microbiology & biotechnology · 2020Article
- The ubiquitin hydrolase Doa4 directly binds Snf7 to inhibit recruitment of ESCRT-III remodeling factors inJournal of cell science · 2020Article
- Extracellular Vesicles and Damage-Associated Molecular Patterns: A Pandora's Box in Health and Disease.Frontiers in immunology · 2020Review
- Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors.The Plant cell · 2019Article
- Endosomal trafficking of yeast membrane proteins.Biochemical Society transactions · 2018Review
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitination directs the sorting of cell surface receptors and other integral membrane proteins into the multivesicular body (MVB) pathway. Cargo proteins are subsequently deubiquitinated before their enclosure within MVB vesicles. In Saccharomyces cerevisiae, Bro1 functions at a late step of MVB sorting and is required for cargo protein deubiquitination. We show that the loss of Bro1 function is suppressed by the overexpression of DOA4, which encodes the ubiquitin thiolesterase required for the removal of ubiquitin from MVB cargoes. Overexpression of DOA4 restores cargo protein deubiquitination and sorting via the MVB pathway and reverses the abnormal endosomal morphology typical of bro1 mutant cells, resulting in the restoration of multivesicular endosomes. We further demonstrate that Doa4 interacts with Bro1 on endosomal membranes and that the recruitment of Doa4 to endosomes requires Bro1. Thus, our results point to a key role for Bro1 in coordinating the timing and location of deubiquitination by Doa4 in the MVB pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.