ArticleInvestigative ophthalmology & visual science2004
Enhanced retinal ganglion cell differentiation by ath5 and NSCL1 coexpression.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Cytoskeleton proteins previously considered exclusive to ganglion cells are transiently expressed by all retinal neuronal precursors.BMC developmental biology · 2011Article
- EBF factors drive expression of multiple classes of target genes governing neuronal development.Neural development · 2011Article
- Generating retinal neurons by reprogramming retinal pigment epithelial cells.Expert opinion on biological therapy · 2010Review
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Pro-photoreceptor activity of chick neurogenin1.Investigative ophthalmology & visual science · 2009Article
- Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.Molecular vision · 2008Article
- Exploring RPE as a source of photoreceptors: differentiation and integration of transdifferentiating cells grafted into embryonic chick eyes.Investigative ophthalmology & visual science · 2006Article
- Pea3 expression is regulated by FGF signaling in developing retina.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Article
- bHLH genes and retinal cell fate specification.Molecular neurobiology · 2005Review
- A role of ath5 in inducing neuroD and the photoreceptor pathway.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Proneural gene ash1 promotes amacrine cell production in the chick retina.Developmental neurobiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeThe molecular mechanism underlying retinal ganglion cell (RGC) differentiation is not fully understood. In this study, the role of the basic helix-loop-helix (bHLH) genes ath5 and NSCL1 in RGC differentiation was examined, by testing whether their coexpression would promote RGC differentiation to a greater extent than either gene alone.
methodsThe replication-competent avian RCAS retrovirus was used to coexpress ath5 and NSCL1 through an internal ribosomal entry site. The effect of the coexpression on RGC differentiation was assayed in vivo in the developing chick retina and in vitro in RPE cell cultures derived from day 6 chick embryos.
resultsCoexpression of ath5 and NSCL1 in RPE cells cultured in the presence of bFGF promoted RPE transdifferentiation toward RGCs, and the degree of transdifferentiation was much higher than with either gene alone. Cells expressing RGC markers, including RA4, calretinin, and two neurofilament-associated proteins, displayed processes that were remarkably long and thin and often had numerous branches, characteristics of long-projecting RGCs. In the developing chick retina, retroviral expression of NSCL1 resulted in a moderate increase in the number of RGCs, results similar to retroviral expression of ath5. Coexpression of ath5 and NSCL1 yielded increases in RGCs greater than the sum of their increases when expressed separately.
conclusionsBoth in vitro and in vivo data indicate that the combination of ath5 and NSCL1 promotes RGC differentiation to a greater degree than either gene alone, suggesting a synergism between ath5 and NSCL1 in advancing RGC development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.