ArticleBiophysical journal2004
Permeation properties of an engineered bacterial OmpF porin containing the EEEE-locus of Ca2+ channels.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Quantitative assessment of porin-mediated solute transport in biomimetic membranes.Applied microbiology and biotechnology · 2025Article
- Dynamics and Interactions of OmpF Porin in an Asymmetric Bacterial Outer Membrane including LPS, ECA, and CPS.Biomacromolecules · 2025Article
- Modeling the Device Behavior of Biological and Synthetic Nanopores with Reduced Models.Entropy (Basel, Switzerland) · 2020Article
- Fosfomycin Permeation through the Outer Membrane Porin OmpF.Biophysical journal · 2019Article
- Outer membrane protein design.Current opinion in structural biology · 2017Review
- A structural study of ion permeation in OmpF porin from anomalous X-ray diffraction and molecular dynamics simulations.Journal of the American Chemical Society · 2013Article
- Interacting ions in biophysics: real is not ideal.Biophysical journal · 2013Review
- Modeling and simulation of ion channels.Chemical reviews · 2012Review
- Ionizable side chains at catalytic active sites of enzymes.European biophysics journal : EBJ · 2012Article
- Computational studies of membrane proteins: models and predictions for biological understanding.Biochimica et biophysica acta · 2012Review
- Single-channel measurements of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli.European biophysics journal : EBJ · 2012Article
- Divalent Metal Ion Transport across Large Biological Ion Channels and Their Effect on Conductance and Selectivity.Biochemistry research international · 2012Article
- Selectivity sequences in a model calcium channel: role of electrostatic field strength.European biophysics journal : EBJ · 2011Article
- Simulations of calcium channel block by trivalent cations: Gd(3+) competes with permeant ions for the selectivity filter.Biochimica et biophysica acta · 2010Article
- The ionization state of D37 in E. coli porin OmpF and the nature of conductance fluctuations in D37 mutants.European biophysics journal : EBJ · 2010Article
- Multiple Scales in the Simulation of Ion Channels and Proteins.The journal of physical chemistry. C, Nanomaterials and interfaces · 2010Article
- Sieving experiments and pore diameter: it's not a simple relationship.European biophysics journal : EBJ · 2010Article
- Energy variational analysis of ions in water and channels: Field theory for primitive models of complex ionic fluids.The Journal of chemical physics · 2010Article
- Understanding ion conductance on a molecular level: an all-atom modeling of the bacterial porin OmpF.Biophysical journal · 2009Article
- Ions and inhibitors in the binding site of HIV protease: comparison of Monte Carlo simulations and the linearized Poisson-Boltzmann theory.Biophysical journal · 2009Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The selectivity filter of the bacterial porin OmpF carries a small net charge close to -1 e and is therefore only slightly cation-selective. Calcium channels, on the other hand, contain four negatively charged glutamates, the EEEE-locus, and are among the most selective cation channels known. We aimed to turn the essentially nonselective OmpF into a Ca2+-selective channel. To that end, two additional glutamates (R42E and R132E) were introduced in the OmpF constriction zone that already contains D113 and E117. Mutant OmpF containing this DEEE-locus has a high Ca2+ over Cl- selectivity and a Na+ current with a strongly increased sensitivity to 1 mM Ca2+. The charge/space competition model, initially applied to the L-type Ca2+ channel, identifies the fixed charge and filter volume as key determinants of ion selectivity, with the precise atomic arrangement having only second-order effects. By implication, the reproduction of fixed charge and filter volume should transform two channels into channels of similar selectivity, even if the two belong to entirely different ion channel families, as is the case for OmpF and the L-type Ca2+ channel. The results presented here fit quite well in the framework of charge/space competition theory.
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