ArticleMethods (San Diego, Calif.)2004
Lipidic cubic phases as matrices for membrane protein crystallization.
Article in Methods (San Diego, Calif.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Biomolecular Crystallisation Through Soft Templates and Seeding.Advances in biochemical engineering/biotechnology · 2026Review
- Structure-based insights into evolution of rhodopsins.Communications biology · 2021Article
- Native MS Analysis of Bacteriorhodopsin and an Empty Nanodisc by Orthogonal Acceleration Time-of-Flight, Orbitrap and Ion Cyclotron Resonance.Analytical chemistry · 2016Article
- Article
- Automating the application of smart materials for protein crystallization.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Membrane proteins, detergents and crystals: what is the state of the art?Acta crystallographica. Section F, Structural biology communications · 2014Review
- Surfactant bilayers maintain transmembrane protein activity.Biophysical journal · 2014Article
- Introduction to protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Review
- Visualization of membrane protein crystals in lipid cubic phase using X-ray imaging.Acta crystallographica. Section D, Biological crystallography · 2013Article
- A Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases.Microfluidics and nanofluidics · 2010Article
- LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment.Biophysical journal · 2010Article
- Determination of the phase diagram for soluble and membrane proteins.The journal of physical chemistry. B · 2010Article
- Effects of impurities on membrane-protein crystallization in different systems.Acta crystallographica. Section D, Biological crystallography · 2009Article
- Microfluidic Generation of Lipidic Mesophases for Membrane Protein Crystallization.Crystal growth & design · 2009Article
- A general protocol for the crystallization of membrane proteins for X-ray structural investigation.Nature protocols · 2009Article
- Structural answers and persistent questions about how nicotinic receptors work.Frontiers in bioscience : a journal and virtual library · 2008Review
- Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization.Current opinion in structural biology · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
This review provides detailed procedures for the crystallization of membrane proteins via the lipidic cubic phase method. Bacteriorhodopsin-specific, hands-on protocols are given for (i) the preparation of bacteriohordopsin from purple membrane by monomerization in octylglucoside and gel filtration chromatography or by selective extraction after pre-treatment with dodecyl-trimethylammonium bromide, (ii) the incorporation of bacteriorhodopsin into lipidic cubic phases by mixing in vials or within coupled syringes and, (iii) the crystallization of bacteriorhodopsin in the lipidic matrix by adding a solid salt or an overlaying with a solution. References for further useful procedures and materials are listed in order to provide biochemists and crystallographers with all information that is necessary to grow crystals of the membrane protein bacteriorhodopsin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.