ArticleHistochemistry and cell biology2004
DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution.
Article in Histochemistry and cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 166 citations in OpenAlex.
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- How to combine the exigencies of histopathological and forensic-genetic investigation: a preliminary study on the use of medicolegal FFPE samples for DNA analysis.Forensic science, medicine, and pathology · 2026Article
- Heat-induced antigen retrieval by using Tris-EDTA solution destroys nuclear structure in certain tissues.Biochemistry and biophysics reports · 2026Article
- Small bowel metastatic SWI/SNF-deficient undifferentiated carcinoma may be predictive of lung primary-a rare presentation with novel SMARCA2 mutation findings in a study of three cases.Virchows Archiv : an international journal of pathology · 2025Article
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- Evaluation and Comparison of Genomic DNA Extraction Methods and PCR Optimization on Archival Formalin-Fixed and Paraffin-Embedded Tissues of Oral Squamous Cell Carcinoma.Diagnostics (Basel, Switzerland) · 2022Article
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- Recurrent Human Papillomavirus-Related Head and Neck Cancer Undergoes Metabolic Reprogramming and Is Driven by Oxidative Phosphorylation.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021Article
- Rejuvenated Vintage Tissue Sections Highlight Individual Antigen Fate During Processing and Long-term Storage.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Consistency of the S5 DNA methylation classifier in formalin-fixed biopsies versus corresponding exfoliated cells for the detection of pre-cancerous cervical lesions.Cancer medicine · 2021Article
- Molecular Analysis of Dirofilaria repens Isolates from Eye-Care Patients in Greece.Acta parasitologica · 2021Article
- A DNA metabarcoding approach for recovering plankton communities from archived samples fixed in formalin.PloS one · 2021Article
- Applying Tobacco, Environmental, and Dietary-Related Biomarkers to Understand Cancer Etiology and Evaluate Prevention Strategies.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2020Article
- Isolation and next generation sequencing of archival formalin-fixed DNA.Journal of anatomy · 2020Article
- A novel approach for extracting DNA from formalin-fixed paraffin-embedded tissue using microwave.Medical journal, Armed Forces India · 2020Article
- Development of a Rapid and Sensitive IDH1/2 Mutation Detection Method for Glial Tumors and a Comparative Mutation Analysis of 236 Glial Tumor Samples.Molecular diagnosis & therapy · 2020Article
- Article
- DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis.World journal of methodology · 2019Article
- Detection of Epidermal Growth Factor Receptor (EGFR) Gene Mutation in Formalin Fixed Paraffin Embedded Tissue by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) in Non-Small Cell Lung Cancer in the Northeastern Region of ThailandAsian Pacific journal of cancer prevention : APJCP · 2019Article
- The mutational landscape of recurrent versus nonrecurrent human papillomavirus-related oropharyngeal cancer.JCI insight · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Based on the antigen retrieval principle, our previous study has demonstrated that heating archival formalin-fixed, paraffin-embedded (FFPE) tissues at a higher temperature and at higher pH value of the retrieval solution may achieve higher efficiency of extracted DNA, when compared to the traditional enzyme digestion method. Along this line of heat-induced retrieval, this further study is focused on development of a simpler and more effective heat-induced DNA retrieval technique by testing various retrieval solutions. Three major experiments using a high temperature heating method to extract DNA from FFPE human lymphoid and other tissue sections were performed to compare: (1) different concentrations of alkaline solution (NaOH or KOH, pH 11.5-12) versus Britton and Robinson type of buffer solution (BR buffer) of pH 12 that was the only retrieval solution tested in our previous study; (2) several chemical solutions (SDS, Tween 20, and GITC of various concentrations) versus BR buffer or alkaline solution; and (3) alkaline solution mixed with chemicals versus BR buffer or single alkaline solution. Efficiency of DNA extraction was evaluated by measuring yields using spectrophotometry, electrophoretic pattern, semiquantitation of tissue dissolution, PCR amplification, and kinetic thermocycling-PCR methods. Results showed that boiling tissue sections in 0.1 M NaOH or KOH or its complex retrieval solutions produced higher yields and better quality of DNA compared to BR buffer or chemical solutions alone. The conclusion was that boiling FFPE tissue sections in 0.1 M alkaline solution is a simpler and more effective heat-induced retrieval protocol for DNA extraction. Combination with some chemicals (detergents) may further significantly improve efficiency of the heat-induced retrieval technique.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.