ArticleImmunogenetics2004
Cloning, expression, and characterization of fugu CD4, the first ectothermic animal CD4.
Article in Immunogenetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 107 citations in OpenAlex.
- CD4 and LAG-3 from sharks to humans: related molecules with motifs for opposing functions.Frontiers in immunology · 2023Article
- IL-2-mTORC1 signaling coordinates the STAT1/T-bet axis to ensure Th1 cell differentiation and anti-bacterial immune response in fish.PLoS pathogens · 2022Article
- Elucidating the Functional Roles of Helper and Cytotoxic T Cells in the Cell-Mediated Immune Responses of Olive Flounder (International journal of molecular sciences · 2021Article
- Characterization of CD4-Positive Lymphocytes in the Antiviral Response of Olive Flounder (International journal of molecular sciences · 2020Article
- Review
- Molecular cloning, expression analysis, and the immune-related role of a thymosin β in the goldfish, Carassius auratus.Fish physiology and biochemistry · 2019Article
- CD4: a vital player in the teleost fish immune system.Veterinary research · 2019Review
- Along the Axis between Type 1 and Type 2 Immunity; Principles Conserved in Evolution from Fish to Mammals.Biology · 2015Review
- First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).PloS one · 2015Article
- Comprehensive analysis of medaka major histocompatibility complex (MHC) class II genes: implications for evolution in teleosts.Immunogenetics · 2013Article
- Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates.BMC evolutionary biology · 2013Article
- Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes.PloS one · 2013Article
- Atlantic salmon reovirus infection causes a CD8 T cell myocarditis in Atlantic salmon (Salmo salar L.).PloS one · 2012Article
- Identification of Treg-like cells in Tetraodon: insight into the origin of regulatory T subsets during early vertebrate evolution.Cellular and molecular life sciences : CMLS · 2011Article
- Phylogenetic and developmental study of CD4, CD8 α and β T cell co-receptor homologs in two amphibian species, Xenopus tropicalis and Xenopus laevis.Developmental and comparative immunology · 2011Article
- A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes.Immunogenetics · 2010Article
- Characterization and expression analysis of CD3varepsilon and CD3gamma/delta in fugu, Takifugu rubripes.Immunogenetics · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have cloned and sequenced the first ectothermic animal CD4 gene from fugu, Takifugu rubripes, using a public database of the third draft sequence of the fugu genome. The fugu CD4 gene encodes a predicted protein of 463 amino acids containing four extracellular immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic tail. Fugu CD4 shares low identity of about 15-20% with avian and mammalian CD4 proteins. Unlike avian and mammalian CD4, fugu CD4 lacks the Cys pair of the first Ig-like domain, but has a unique possible disulfide bond in the third domain. These differences suggest that fugu CD4 may have a different structure that could affect binding of major histocompatibility complex class II molecules and subsequent T-cell activation. In the putative fugu cytoplasmic region, the protein tyrosine kinase p56lck binding motif is conserved. The predicted fugu CD4 gene is composed of 12 exons, differing from other CD4 genes, but showing conserved synteny and many conserved sequence motifs in the promoter region. RT-PCR analysis demonstrated that the fugu CD4 gene is expressed predominantly in lymphoid tissues. We also show that fugu CD4 can be expressed on the surface of cells via transfection. Molecular characterization of CD4 in fish provides insights into the evolution of both the CD4 molecule and the immune system.
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