ArticleJournal of virology1992
Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S.
Article in Journal of virology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Loss of keratin 14 expression from immortalized keratinocytes by promoter methylation.Experimental dermatology · 2024Article
- Cell Immortality:Life (Basel, Switzerland) · 2024Review
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- Opposing oncogenic activities of small DNA tumor virus transforming proteins.Trends in microbiology · 2011Review
- Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human papillomaviruses suppress cell transformation through interaction with FOXK1/K2 transcription factors.Journal of virology · 2010Article
- Interaction of MEQ protein and C-terminal-binding protein is critical for induction of lymphomas by Marek's disease virus.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification of genes involved in epithelial-mesenchymal transition and tumor progression.Oncogene · 2001Article
- Physical and functional interactions between the corepressor CtBP and the Epstein-Barr virus nuclear antigen EBNA3C.Journal of virology · 2001Article
- Article
- Expression of the pRb-binding regions of E1A enables efficient transformation of primary epithelial cells by v-src.Journal of virology · 1998Article
- Role of p300-family proteins in E1A oncogene induction of cytolytic susceptibility and tumor cell rejection.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Efficient nuclear localization and immortalizing ability, two functions dependent on the adenovirus type 5 (Ad5) E1A second exon, are necessary for cotransformation with Ad5 E1B but not with T24ras.Journal of virology · 1995Article
- Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Induction of gene expression by exon 2 of the major E1A proteins of adenovirus type 5.Journal of virology · 1993Article
- Adenovirus-E1A proteins transform cells by sequestering regulatory proteins.Molecular biology reports · 1993Review
- Domains of E1A that bind p105Rb, p130, and p300 are required to block nerve growth factor-induced neurite growth in PC12 cells.Molecular biology of the cell · 1993Article
- Article
- The adenovirus E1A 12S product displays functional redundancy in activating the human proliferating cell nuclear antigen promoter.Journal of virology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immortalization of primary cells is a multistep process. The adenovirus E1A 12S gene product is a member of the class of oncoproteins that have the ability to establish primary cells as cell lines in culture. It is encoded by two exons. Extensive mutational analysis demonstrates that four regions of the E1A 12S gene, encoded by both exons, are necessary for immortalization of primary epithelial cells. Expression of two regions is necessary to activate quiescent cells into the cell cycle but is unable to extend the life span of these cells in culture and thus cannot immortalize them. These regions are encoded by the first exon. A third first-exon region, for which no function has yet been identified, is also required. These three regions are also required for 12S to cooperate with an activated ras gene to bring about tumorigenic transformation. The fourth region is required to maintain the cells in a proliferative mode, extend their life span in culture, and induce an autocrine growth factor. These functions are encoded by the second exon. The cells immortalized by wild-type 12S and immortalization-competent mutants retain their epithelial morphology and expression of keratin and vimentin intermediate filament proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.