ArticleInfection and immunity2004
Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids.Molecular oral microbiology · 2026Article
- Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.Molecular oral microbiology · 2023Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Genetic Manipulations of Oral Spirochete Treponema denticola.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.Applied and environmental microbiology · 2016Article
- A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405.Applied and environmental microbiology · 2015Article
- Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.Applied and environmental microbiology · 2015Article
- Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.Journal of bacteriology · 2015Article
- Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.Applied and environmental microbiology · 2012Article
- Treponema denticola interactions with host proteins.Journal of oral microbiology · 2012Article
- Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.Applied and environmental microbiology · 2011Article
- Identification of the primary mechanism of complement evasion by the periodontal pathogen, Treponema denticola.Molecular oral microbiology · 2011Article
- A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.Journal of microbiological methods · 2010Article
- Differential regulation of the multiple flagellins in spirochetes.Journal of bacteriology · 2010Article
- Native cellular architecture of Treponema denticola revealed by cryo-electron tomography.Journal of structural biology · 2008Article
- Heterologous expression of the Treponema pallidum laminin-binding adhesin Tp0751 in the culturable spirochete Treponema phagedenis.Journal of bacteriology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments. Wild-type fliG genes from T. denticola and from Treponema pallidum were cloned into this expression plasmid. In both cases, the gene restored the ability of the mutant to gyrate its cell ends and enabled colony spreading in agarose. This shuttle plasmid enables high-level expression of genes in T. denticola and possesses an efficient selectable marker that provides a new tool for treponemal genetics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.