ArticleInfection and immunity2004
The cyclic AMP-dependent protein kinase a network regulates development and virulence in Aspergillus fumigatus.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 170 citations in OpenAlex.
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- Research advances and public health strategies in China on WHO priority fungal pathogens.Mycology · 2025Review
- Integrated multi-omics identifies pathways governing interspecies interaction between A. fumigatus and K. pneumoniae.Communications biology · 2024Article
- Functional analysis of the Aspergillus fumigatus kinome identifies a druggable DYRK kinase that regulates septal plugging.Nature communications · 2024Article
- Promising immunotherapeutic targets for treating candidiasis.Frontiers in cellular and infection microbiology · 2024Review
- Pathogenicity and virulence ofVirulence · 2023Review
- Research on the Molecular Interaction Mechanism between Plants and Pathogenic Fungi.International journal of molecular sciences · 2022Review
- Functional analysis of seven regulators of G protein signaling (RGSs) in the nematode-trapping fungusVirulence · 2021Article
- Discovery of fungal surface NADases predominantly present in pathogenic species.Nature communications · 2021Article
- CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster inFrontiers in microbiology · 2021Article
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- Heterotrimeric G-protein signalers and RGSs inPathogens (Basel, Switzerland) · 2020Review
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27 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus fumigatus is an important pathogen of immunocompromised hosts, causing pneumonia and invasive disseminated disease with high mortality. To determine the importance of the cyclic AMP (cAMP) signaling pathway for virulence, the pkaC1 gene encoding a protein kinase A (PKA) catalytic subunit was cloned and characterized. Deletion of pkaC1 led to reduced conidiation and growth. PKA activity was not detectable in DeltapkaC1, DeltagpaB, and DeltaacyA mutant strains. gpaB and acyA encode a G protein alpha subunit involved in cAMP signal transduction and adenylate cyclase, respectively. Addition of cAMP led to PKA activity in crude extracts of both the DeltagpaB and DeltaacyA strains but not in crude extracts of the DeltapkaC1 strain. These findings provide evidence that PKAC1 represents the predominant form of PKA under the conditions tested, and GPAB and ACYA are members of the cAMP signaling cascade. Analysis of a pksPp-lacZ gene fusion indicated that the expression of the pathogenicity determinant-encoding pksP gene was reduced in DeltapkaC1 mutant strains compared with the expression of the gene fusion in the parental strain. In a low-dose murine inhalation model, conidia of both the DeltapkaC1 and DeltagpaB mutant strains were almost avirulent. Taken together, these findings indicate that the cAMP-PKA signal transduction pathway is required for A. fumigatus pathogenicity.
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