ArticleInfection and immunity2004
Contribution of Burkholderia cenocepacia flagella to infectivity and inflammation.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 84 citations in OpenAlex.
- Review
- Convergence of Hypervirulence and Multidrug-Resistance inInfection and drug resistance · 2024Article
- The Global Regulator MftR Controls Virulence and Siderophore Production in Burkholderia thailandensis.Journal of bacteriology · 2022Article
- Evolution towards Virulence in amBio · 2021Article
- Advances in Phage Therapy: Targeting theViruses · 2021Review
- Flagellin FromFrontiers in immunology · 2021Article
- Curcumin β-D-Glucuronide Modulates an Autoimmune Model of Multiple Sclerosis with Altered Gut Microbiota in the Ileum and Feces.Frontiers in cellular and infection microbiology · 2021Article
- Interferon-gamma-activated macrophages infected with Burkholderia cenocepacia process and present bacterial antigens to T-cells by class I and II major histocompatibility complex molecules.Emerging microbes & infections · 2020Article
- TLRs in pulmonary diseases.Life sciences · 2019Review
- Genetic Determinants Associated WithFrontiers in microbiology · 2018Article
- Article
- Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection.Virulence · 2017Article
- Macrophages, but not neutrophils, are critical for proliferation of Burkholderia cenocepacia and ensuing host-damaging inflammation.PLoS pathogens · 2017Article
- Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa.Infection and immunity · 2017Article
- NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111.PloS one · 2017Article
- An Oxygen-Sensing Two-Component System in the Burkholderia cepacia Complex Regulates Biofilm, Intracellular Invasion, and Pathogenicity.PLoS pathogens · 2017Article
- Crosstalk between sugarcane and a plant-growth promoting Burkholderia species.Scientific reports · 2016Article
- Review
- Synthetic Cystic Fibrosis Sputum Medium Regulates Flagellar Biosynthesis through the flhF Gene in Burkholderia cenocepacia.Frontiers in cellular and infection microbiology · 2016Article
- Identification of Poly-N-acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm Matrix.The Journal of biological chemistry · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Burkholderia cenocepacia is an opportunistic pathogen that can cause severe lung infections in cystic fibrosis patients. To understand the contribution of B. cenocepacia flagella to infection, a strain mutated in the major flagellin subunit, fliCII, was constructed in B. cenocepacia K56-2 and tested in a murine agar bead model of lung infection. C57/BL6 mice infected with approximately 10(8) wild-type K56-2 bacteria exhibited 40% mortality after 3 days, whereas no mortality was noted in mice infected with the fliCII mutant. Among the mice surviving the infection with either strain, there was no significant difference in the bacterial loads in the lungs and spleen, bacteremia, weight loss, or infiltration of immune effector cells at 3 days postinfection. Similar results were observed at 24 h, prior to expression of the lethality phenotype. KC, a murine interleukin-8 (IL-8) homolog, was elevated in both the bronchoalveolar lavage fluid and serum of mice infected with the wild type compared to the fliCII mutant at 24 h, suggesting that flagella stimulated host cells. To demonstrate that flagella contributed to these responses, the interaction between B. cenocepacia and Toll-like receptor 5 (TLR5) was investigated. Infection of HEK293 cells with heat-killed wild-type K56-2, but not infection with the fliCII mutant, resulted in both NF-kappaB activation and IL-8 secretion that was dependent upon expression of TLR5. Together, these results demonstrate that B. cenocepacia flagella contribute to virulence in an in vivo infection model, and that induction of host immune responses through interaction with TLR5 may contribute to its overall pathogenic potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.