ArticleBMC cancer2004
Sensitization of interferon-gamma induced apoptosis in human osteosarcoma cells by extracellular S100A4.
Article in BMC cancer, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Constructing a ferroptosis-related prognostic model for osteosarcoma based on scRNA-seq dataset and WGCNA analysis.International journal of clinical and experimental pathology · 2025Article
- S100A4 contributes to colorectal carcinoma aggressive behavior and to chemoradiotherapy resistance in locally advanced rectal carcinoma.Scientific reports · 2024Article
- Protective effect of cilostazol and verapamil against thioacetamide-induced hepatotoxicity in rats may involve Nrf2/GSK-3β/NF-κB signaling pathway.Toxicology research · 2022Article
- Interferon gamma induces protective non-canonical signaling pathways in primary neurons.Journal of neurochemistry · 2015Article
- S100 protein family in human cancer.American journal of cancer research · 2014Review
- S100 proteins as diagnostic and prognostic markers in colorectal and hepatocellular carcinoma.Hepatitis monthly · 2012Article
- S100A4 in esophageal cancer: is this the one to blame?World journal of gastroenterology · 2012Article
- S100A4 expression in xenograft tumors of human carcinoma cell lines is induced by the tumor microenvironment.The American journal of pathology · 2011Article
- The metastasis-promoting protein S100A4 regulates mammary branching morphogenesis.Developmental biology · 2011Article
- Article
- Signal transduction mechanisms involved in S100A4-induced activation of the transcription factor NF-kappaB.BMC cancer · 2010Article
- S100A4 and metastasis: a small actor playing many roles.The American journal of pathology · 2010Review
- The metastasis-associated protein S100A4 exists in several charged variants suggesting the presence of posttranslational modifications.BMC cancer · 2008Article
- S100A4: a common mediator of epithelial-mesenchymal transition, fibrosis and regeneration in diseases?Journal of molecular medicine (Berlin, Germany) · 2008Review
- The metastasis associated protein S100A4: role in tumour progression and metastasis.British journal of cancer · 2005Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundS100A4 is a small Ca2+-binding protein of the S100 family with metastasis-promoting properties. Recently, secreted S100A4 protein has been shown to possess a number of functions, including induction of angiogenesis, stimulation of cell motility and neurite extension.
methodsCell cultures from two human osteosarcoma cell lines, OHS and its anti-S100A4 ribozyme transfected counterpart II-11b, was treated with IFN-gamma and recombinant S100A4 in order to study the sensitizing effects of extracellular S100A4 on IFN-gamma mediated apoptosis. Induction of apoptosis was demonstrated by DNA fragmentation, cleavage of poly (ADP-ribose) polymerase and Lamin B.
resultsIn the present work, we found that the S100A4-expressing human osteosarcoma cell line OHS was more sensitive to IFN-gamma-mediated apoptosis than the II-11b cells. S100A4 protein was detected in conditioned medium from OHS cells, but not from II-11b cells, and addition of recombinant S100A4 to the cell medium sensitized II-11b cells to apoptosis induced by IFN-gamma. The S100A4/IFN-gamma-mediated induction of apoptosis was shown to be independent of caspase activation, but dependent on the formation of reactive oxygen species. Furthermore, addition of extracellular S100A4 was demonstrated to activate nuclear factor-kappa B (NF-kappa B).
conclusionIn conclusion, we have shown that S100A4 sensitizes osteosarcoma cells to IFN-gamma-mediated induction of apoptosis. Additionally, extracellular S100A4 activates NF-kappa B, but whether these events are causally related remains unknown.
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