ArticleRNA (New York, N.Y.)2004
A stop codon-dependent internal secondary translation initiation region in Escherichia coli rpoS.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Advances in Decoding Bacterial N-Terminal Proteoforms: Technologies, Challenges, and Functional Insights.Microorganisms · 2026Review
- Genes within Genes in Bacterial Genomes.Microbiology spectrum · 2018Review
- Comparative Proteomics Enables Identification of Nonannotated Cold Shock Proteins in E. coli.Journal of proteome research · 2017Article
- Expression and purification of recombinant Saccharomyces cerevisiae mitochondrial carrier protein YGR257Cp (Mtm1p).Protein expression and purification · 2014Article
- Additional pathway to translate the downstream ndhK cistron in partially overlapping ndhC-ndhK mRNAs in chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The constancy of global regulation across a species: the concentrations of ppGpp and RpoS are strain-specific in Escherichia coli.BMC microbiology · 2011Article
- E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.PLoS genetics · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sigma S (sigmaS) encoded by rpoS is a stationary phase-specific sigma subunit of the Escherichia coli RNA polymerase holoenzyme. In many E. coli strains, rpoS has an amber stop as codon 33 (rpoSAm), resulting in a 32-amino-acid-long peptide. Nevertheless, suppressor-free rpoSAm strains have functional sigmaS. This led us to hypothesize the presence of an intracistronic secondary translational initiation region (STIR) in the E. coli rpoS gene. Here, we demonstrate that the STIR is functional and is controlled by the upstream amber stop codon 33. Removal of the primary translational initiation region did not abolish translation from STIR, ruling out translational coupling. Importantly, the functional STIR conferred survival advantage. Taken together, our results reveal a hitherto unknown physiologically significant post-transcriptional process in E. coli rpoSAm strains.
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