ArticleMolecular biology of the cell2004
Toc12, a novel subunit of the intermembrane space preprotein translocon of chloroplasts.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 115 citations in OpenAlex.
- Functional Interaction Between Toc75 POTRA1 Domain and Tic22-III in the Intermembrane Space During Chloroplast Protein Import.Plant direct · 2026Article
- Genetic Mapping of theFrontiers in plant science · 2022Article
- Article
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
- Molecular characterization and expression analysis of chloroplast protein import components in tomato (Solanum lycopersicum).PloS one · 2014Article
- Evolutionary, molecular and genetic analyses of Tic22 homologues in Arabidopsis thaliana chloroplasts.PloS one · 2013Article
- Protein import into the photosynthetic organelles ofSymbiosis (Philadelphia, Pa.) · 2012Article
- Tic22 is an essential chaperone required for protein import into the apicoplast.The Journal of biological chemistry · 2012Article
- Structure and conservation of the periplasmic targeting factor Tic22 protein from plants and cyanobacteria.The Journal of biological chemistry · 2012Article
- Overproduction, purification and crystallization of PfTic22, a component of the import apparatus from the apicoplast of Plasmodium falciparum.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor.PLoS pathogens · 2011Article
- The Plastid Outer Envelope - A Highly Dynamic Interface between Plastid and Cytoplasm.Frontiers in plant science · 2011Article
- Common ground for protein translocation: access control for mitochondria and chloroplasts.Nature reviews. Molecular cell biology · 2011Review
- More membranes, more proteins: complex protein import mechanisms into secondary plastids.Protist · 2010Review
- Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma.Plant physiology · 2010Article
- Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts.The Plant cell · 2010Article
- Joining forces: the interface of gravitropism and plastid protein import.Plant signaling & behavior · 2009Article
- OWL1: an Arabidopsis J-domain protein involved in perception of very low light fluences.The Plant cell · 2009Article
- A 1-megadalton translocation complex containing Tic20 and Tic21 mediates chloroplast protein import at the inner envelope membrane.The Plant cell · 2009Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translocation of proteins across membranes is essential for the biogenesis of each cell and is achieved by proteinaceous complexes. We analyzed the translocation complex of the intermembrane space from chloroplasts and identified a 12-kDa protein associated with the Toc machinery. Toc12 is an outer envelope protein exposing a soluble domain into the intermembrane space. Toc12 contains a J-domain and stimulates the ATPase activity of DnaK. The conformational stability and the ability to stimulate Hsp70 are dependent on a disulfide bridge within the loop region of the J-domain, suggesting a redox-regulated activation of the chaperone. Toc12 is associated with Toc64 and Tic22. Its J-domain recruits the Hsp70 of outer envelope membrane to the intermembrane space translocon and facilitates its interaction to the preprotein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.