ArticleJournal of bacteriology2004
Cloning and expression of afpA, a gene encoding an antifreeze protein from the arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 93 citations in OpenAlex.
- Relevance of Microorganisms in Causing Rain and Snow.Recent patents on biotechnology · 2025Review
- Cold adaptedFrontiers in microbiology · 2023Review
- Antifreeze Proteins: A Tale of Evolution From Origin to Energy Applications.Frontiers in bioengineering and biotechnology · 2021Review
- LeveragingFrontiers in microbiology · 2021Review
- A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering.3 Biotech · 2019Review
- Draft genome sequences of bacteria isolated from the Deschampsia antarctica phyllosphere.Extremophiles : life under extreme conditions · 2018Article
- Adaptational properties and applications of cold-active lipases from psychrophilic bacteria.Extremophiles : life under extreme conditions · 2015Review
- Bacterial ice crystal controlling proteins.Scientifica · 2014Review
- Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12.Extremophiles : life under extreme conditions · 2013Article
- Bacterial gene expression at low temperatures.Extremophiles : life under extreme conditions · 2012Review
- Plant growth-promoting bacteria: mechanisms and applications.Scientifica · 2012Review
- Antifreeze protein in Antarctic marine diatom, Chaetoceros neogracile.Marine biotechnology (New York, N.Y.) · 2010Article
- Direct visualization of spruce budworm antifreeze protein interacting with ice crystals: basal plane affinity confers hyperactivity.Biophysical journal · 2008Article
- Fluorescence microscopy evidence for quasi-permanent attachment of antifreeze proteins to ice surfaces.Biophysical journal · 2007Article
- Psychrophilic microorganisms: challenges for life.EMBO reports · 2006Review
- Freeze-thaw tolerance and clues to the winter survival of a soil community.Applied and environmental microbiology · 2006Article
- Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was cloned by PCR and inverse PCR using primers designed from partial amino acid sequences of the isolated AFP. The predicted gene product, AfpA, had a molecular mass of 47.3 kDa, a pI of 3.51, and no previously known function. Although AfpA is a secreted protein, it lacked an N-terminal signal peptide and was shown by sequence analysis to have two possible secretion systems: a hemolysin-like, calcium-binding secretion domain and a type V autotransporter domain found in gram-negative bacteria. Expression of afpA in Escherichia coli yielded an intracellular 72-kDa protein modified with both sugars and lipids that exhibited lower levels of antifreeze and ice nucleation activities than the native protein. The 164-kDa AFP previously purified from P. putida GR12-2 was a lipoglycoprotein, and the carbohydrate was required for ice nucleation activity. Therefore, the recombinant protein may not have been properly posttranslationally modified. The AfpA sequence was most similar to cell wall-associated proteins and less similar to ice nucleation proteins (INPs). Hydropathy plots revealed that the amino acid sequence of AfpA was more hydrophobic than those of the INPs in the domain that forms the ice template, thus suggesting that AFPs and INPs interact differently with ice. To our knowledge, this is the first gene encoding a protein with both antifreeze and ice nucleation activities to be isolated and characterized.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.