ArticleJournal of bacteriology2004
The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 74 citations in OpenAlex.
- Regulatory roles of the LysR-type transcriptional regulator GltC in metabolism, stress tolerance, and virulence ofMicrobiology spectrum · 2026Article
- The Transcriptional Regulator Crt1 is Involved in The Pathogenic Lifestyle ofPolish journal of microbiology · 2026Article
- LysR-Type Transcriptional Regulator VirR Responds to Temperature and pH and Directly Activates the Transcription ofInternational journal of microbiology · 2025Article
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- The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis ofAntimicrobial agents and chemotherapy · 2024Article
- First report on in-depth genome and comparative genome analysis of a metal-resistant bacteriumFrontiers in microbiology · 2024Article
- Catabolite control protein C contributes to virulence and hydrogen peroxide-induced oxidative stress responses inFrontiers in microbiology · 2024Article
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- Fine-tuning gene expression of regulator AdmX for improved biosynthesis of andrimid in Erwinia persicina BST187.Applied microbiology and biotechnology · 2023Article
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- An Autobioluminescent Method for EvaluatingMicrobiology spectrum · 2022Article
- Identification of genes required for the fitness of Rhodococcus equi during the infection of mice via signature-tagged transposon mutagenesis.The Journal of veterinary medical science · 2021Article
- LeuO, a LysR-Type Transcriptional Regulator, Is Involved in Biofilm Formation and Virulence ofFrontiers in cellular and infection microbiology · 2021Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- Article
- Contributions of a LysR Transcriptional Regulator to Listeria monocytogenes Virulence and Identification of Its Regulons.Journal of bacteriology · 2020Article
- Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens.Microbiology and molecular biology reviews : MMBR · 2018Review
- Regulation of the aceI multidrug efflux pump gene in Acinetobacter baumannii.The Journal of antimicrobial chemotherapy · 2018Article
- Perturbation of the metabolic network in Salmonella enterica reveals cross-talk between coenzyme A and thiamine pathways.PloS one · 2018Article
- The Rhodococcus equi virulence protein VapA disrupts endolysosome function and stimulates lysosome biogenesis.MicrobiologyOpen · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The virulence of the intracellular pathogen Rhodococcus equi in foals is dependent on the presence of an 81-kb virulence plasmid encoding the virulence protein VapA. Expression of this protein is induced by exposure to oxidative stress, high temperatures, and low pHs, which reflect the conditions encountered by R. equi when it enters the host environment. The aim of this study was to determine whether the LysR-type transcriptional regulator VirR, which is encoded by the virulence plasmid, is required for the expression of vapA. It was shown that the virR gene is cotranscribed with four downstream genes, one of which encodes a two-component response regulator. The expression of VapA, as monitored by Western blotting, was completely dependent on the presence of virR. Maximal expression was observed when vapA was present together with the complete virR operon, suggesting that at least one of the virR operon genes, in addition to virR, is required for the expression of vapA to wild-type levels. The transcriptional start site of vapA was determined to be a cytidine located 226 bp upstream from the vapA initiation codon. His-tagged VirR protein was expressed in Escherichia coli and purified by nickel affinity chromatography. DNA binding studies showed that purified VirR binds to a DNA fragment containing the vapA promoter. We therefore conclude that VirR is required for the activation of vapA transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.