ArticlePlant molecular biology2004
Monitoring of gene expression profiles and isolation of candidate genes involved in pollination and fertilization in rice ( Oryza sativa L.) with a 10K cDNA microarray.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 79 citations in OpenAlex.
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- Stigmatic Transcriptome Analysis of Self-Incompatible and Compatible Pollination inFrontiers in plant science · 2022Article
- Comparative transcriptome analysis during seeds development between two soybean cultivars.PeerJ · 2021Article
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- A novel signal transduction pathway that modulates rhl quorum sensing and bacterial virulence in Pseudomonas aeruginosa.PLoS pathogens · 2014Article
- Characterization of Osmads6-5, a null allele, reveals that OsMADS6 is a critical regulator for early flower development in rice (Oryza sativa L.).Plant molecular biology · 2012Article
- Labellum transcriptome reveals alkene biosynthetic genes involved in orchid sexual deception and pollination-induced senescence.Functional & integrative genomics · 2012Article
- Expression dynamics of metabolic and regulatory components across stages of panicle and seed development in indica rice.Functional & integrative genomics · 2012Article
- Transcriptomic analysis of rice (Oryza sativa) developing embryos using the RNA-Seq technique.PloS one · 2012Article
- Pollen-specific expression of Oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and Arabidopsis transgenic systems.Molecular biotechnology · 2011Article
- Whole genome analysis of gene expression reveals coordinated activation of signaling and metabolic pathways during pollen-pistil interactions in Arabidopsis.Plant physiology · 2011Article
- Cytoplasmic male sterility-regulated novel microRNAs from maize.Functional & integrative genomics · 2011Article
- Modulation of transcription factor and metabolic pathway genes in response to water-deficit stress in rice.Functional & integrative genomics · 2011Article
- Transcription profiling of fertilization and early seed development events in a solanaceous species using a 7.7 K cDNA microarray from Solanum chacoense ovules.BMC plant biology · 2010Article
- Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.).Journal of experimental botany · 2010Article
- Genome-wide analysis of gene expression profiles during ear development of maize.Plant molecular biology · 2009Article
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- Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray.Plant & cell physiology · 2008Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To monitor gene expression profiles during pollination and fertilization in rice at a genome scale, we generated 73,424 high-quality expressed sequence tags (ESTs) derived from the green/etiolated shoot and pistil (0-5 h after pollination, 5hP) of rice, which were subsequently used to construct a cDNA microarray containing ca. 10 000 unique rice genes. This microarray was used to analyze gene expression in pistil unpollinated (UP), 5hP and 5DAP(5 days after pollination), anther, shoot, root, 10-day-old embryo (10EM) and 10-day-old endosperm (10EN). Clustering analysis revealed that the anther has a gene-expression profile more similar to root than to pistil and most pistil-preferentially expressed genes respond to pollination and/or fertilization. There are 253 ESTs exhibiting differential expression (e +/- 2-fold changes) during pollination and fertilization, and about 70% of them can be assigned a putative function. We also recovered 20 genes similar to pollination-related and/or fertility-related genes previously identified as well as genes that were not implicated previously. Microarray and real-time PCR analyses showed that the array sensitivity was estimated at 1-5 copies of mRNA per cell, and the differentially expressed genes showed a high correlation between the two methods. Our results indicated that this cDNA microarray constructed here is reliable and can be used for monitoring gene expression profiles in rice. In addition, the genes that differentially expressed during pollination represent candidate genes for dissecting molecular mechanism of this important biological process in rice.
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