ArticlePLoS biology2004
The regenerative plasticity of isolated urodele myofibers and its dependence on MSX1.
Article in PLoS biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 106 citations in OpenAlex.
- Epigenetic aging and rejuvenation of the brain: drivers, consequences, and interventions.BMB reports · 2026Review
- Amphibian-derived wound healing peptides: chemical molecular treasure trove for skin wound treatment.Frontiers in pharmacology · 2023Review
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- Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential.Developmental biology · 2018Article
- Elixir of Life: Thwarting Aging With Regenerative Reprogramming.Circulation research · 2018Review
- Mechanisms of urodele limb regeneration.Regeneration (Oxford, England) · 2017Review
- p53 on the crossroad between regeneration and cancer.Cell death and differentiation · 2017Review
- Lizard tail skeletal regeneration combines aspects of fracture healing and blastema-based regeneration.Development (Cambridge, England) · 2016Article
- Human regeneration: An achievable goal or a dream?Journal of biosciences · 2016Review
- Regeneration versus scarring in vertebrate appendages and heart.The Journal of pathology · 2016Review
- Turning terminally differentiated skeletal muscle cells into regenerative progenitors.Nature communications · 2015Article
- Article
- Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability.Stem cells (Dayton, Ohio) · 2014Article
- Keeping at arm's length during regeneration.Developmental cell · 2014Review
- Molecular pathways of pannexin1-mediated neurotoxicity.Frontiers in physiology · 2014Review
- De novo transcriptome sequencing of axolotl blastema for identification of differentially expressed genes during limb regeneration.BMC genomics · 2013Article
- Biochemical and mechanical environment cooperatively regulate skeletal muscle regeneration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- The cell biology of regeneration.The Journal of cell biology · 2012Review
- Vertebrate-like regeneration in the invertebrate chordate amphioxus.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conversion of multinucleate postmitotic muscle fibers to dividing mononucleate progeny cells (cellularisation) occurs during limb regeneration in salamanders, but the cellular events and molecular regulation underlying this remarkable process are not understood. The homeobox gene Msx1 has been studied as an antagonist of muscle differentiation, and its expression in cultured mouse myotubes induces about 5% of the cells to undergo cellularisation and viable fragmentation, but its relevance for the endogenous programme of salamander regeneration is unknown. We dissociated muscle fibers from the limb of larval salamanders and plated them in culture. Most of the fibers were activated by dissociation to mobilise their nuclei and undergo cellularisation or breakage into viable multinucleate fragments. This was followed by microinjection of a lineage tracer into single fibers and analysis of the labelled progeny cells, as well as by time-lapse microscopy. The fibers showing morphological plasticity selectively expressed Msx1 mRNA and protein. The uptake of morpholino antisense oligonucleotides directed to Msx1 led to a specific decrease in expression of Msx1 protein in myonuclei and marked inhibition of cellularisation and fragmentation. Myofibers of the salamander respond to dissociation by activation of an endogenous programme of cellularisation and fragmentation. Lineage tracing demonstrates that cycling mononucleate progeny cells are derived from a single myofiber. The induction of Msx1 expression is required to activate this programme. Our understanding of the regulation of plasticity in postmitotic salamander cells should inform strategies to promote regeneration in other contexts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.