ArticleNucleic acids research2004
Mos as a tool for genome-wide insertional mutagenesis in Caenorhabditis elegans: results of a pilot study.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Further evidence for the variability of the 18S rDNA loci in the family Tingidae (Hemiptera, Heteroptera).Comparative cytogenetics · 2016Article
- Forward and reverse mutagenesis in C. elegans.WormBook : the online review of C. elegans biology · 2014Review
- Induction of cap-independent BiP (hsp-3) and Bcl-2 (ced-9) translation in response to eIF4G (IFG-1) depletion in C. elegans.Translation (Austin, Tex.) · 2014Article
- Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.Nature methods · 2013Article
- Transmembrane protein OSTA-1 shapes sensory cilia morphology via regulation of intracellular membrane trafficking in C. elegans.Development (Cambridge, England) · 2013Article
- NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins.Nature neuroscience · 2012Article
- A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.PloS one · 2012Article
- Patterns of rDNA chromosomal localization in Palearctic Cephalota and Cylindera (Coleoptera: Carabidae: Cicindelini) with different numbers of X-chromosomes.Comparative cytogenetics · 2011Article
- EGG molecules couple the oocyte-to-embryo transition with cell cycle progression.Results and problems in cell differentiation · 2011Review
- Saturation of the human phenome.Current genomics · 2010Article
- Reverse genetics in eukaryotes.Biology of the cell · 2010Review
- C. elegans SWAN-1 Binds to EGL-9 and regulates HIF-1-mediated resistance to the bacterial pathogen Pseudomonas aeruginosa PAO1.PLoS pathogens · 2010Article
- Article
- Target site selection by the mariner-like element, Mos1.Genetica · 2010Article
- Site-directed integration of transgenes: transposons revisited using DNA-binding-domain technologies.Genetica · 2010Article
- Two distinct roles for EGL-9 in the regulation of HIF-1-mediated gene expression in Caenorhabditis elegans.Genetics · 2009Article
- Mariner transposons as genetic tools in vertebrate cells.Genetica · 2009Review
- The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans.Genetica · 2009Review
- The bacterial Tn9 chloramphenicol resistance gene: an attractive DNA segment for Mos1 mariner insertions.Molecular genetics and genomics : MGG · 2009Article
- The Caenorhabditis elegans ing-3 gene regulates ionizing radiation-induced germ-cell apoptosis in a p53-associated pathway.Genetics · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sequence of the Caenorhabditis elegans genome contains approximately 19 000 genes. Available mutants currently exist for <20% of these genes. The existence of a Mos-based inducible transposon system in C.elegans could theoretically serve as a tool to saturate the genome with insertions. We report here the results of a pilot study aimed at assaying this strategy. We generated 914 independent random Mos insertions and determined their location by inverse PCR. The distribution of the insertions throughout the genome does not reveal any gross distortion, with the exception of a major hotspot on chromosome I (rDNA locus). Transposons are evenly distributed between the genic and intergenic regions. Within genes, transposons insert preferentially into the introns. We derived the consensus target site for Mos in C.elegans (ATATAT), which is common to Tc1, another mariner element. Finally, we assayed the mutagenic properties of insertions located in exons by comparing the phenotype of homozygous strains to that of known mutations or RNAi of the same gene. This pilot experiment shows that a Mos-based approach is a viable strategy that can contribute to the constitution of genome-wide collections of identified C.elegans mutants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.