ArticlePlant physiology2004
Dynamic changes in the osmotic water permeability of protoplast plasma membrane.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 86 citations in OpenAlex.
- How sterols affect protoplasts plasma membrane water permeability and their volume under osmotic shock.Journal of plant research · 2025Article
- Out of the blue: Phototropins of the leaf vascular bundle sheath mediate the regulation of leaf hydraulic conductance by blue light.The Plant cell · 2022Article
- Cell membrane fluidity and ROS resistance define DMSO tolerance of cryopreserved synovial MSCs and HUVECs.Stem cell research & therapy · 2022Article
- Experimental and conceptual approaches to root water transport.Plant and soil · 2022Review
- Plasma membrane aquaporins interact with the endoplasmic reticulum resident VAP27 proteins at ER-PM contact sites and endocytic structures.The New phytologist · 2020Article
- Modification of the Expression of the Aquaporin ZmPIP2;5 Affects Water Relations and Plant Growth.Plant physiology · 2020Article
- Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Biotechnology for biofuels · 2019Article
- Brain Transport Profiles of Ginsenoside RbFrontiers in pharmacology · 2018Article
- Article
- The Water to Solute Permeability Ratio Governs the Osmotic Volume Dynamics in Beetroot Vacuoles.Frontiers in plant science · 2016Article
- Do phosphoinositides regulate membrane water permeability of tobacco protoplasts by enhancing the aquaporin pathway?Planta · 2015Article
- The role of plasma membrane aquaporins in regulating the bundle sheath-mesophyll continuum and leaf hydraulics.Plant physiology · 2014Article
- Measuring the osmotic water permeability coefficient (Pf) of spherical cells: isolated plant protoplasts as an example.Journal of visualized experiments : JoVE · 2014Article
- Arabidopsis SNAREs SYP61 and SYP121 coordinate the trafficking of plasma membrane aquaporin PIP2;7 to modulate the cell membrane water permeability.The Plant cell · 2014Article
- Human AQP1 is a constitutively open channel that closes by a membrane-tension-mediated mechanism.Biophysical journal · 2013Article
- Regulation of leaf hydraulics: from molecular to whole plant levels.Frontiers in plant science · 2013Article
- Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121.The Plant cell · 2012Article
- Aquaporin tetramer composition modifies the function of tobacco aquaporins.The Journal of biological chemistry · 2010Article
- Intracellular pH sensing is altered by plasma membrane PIP aquaporin co-expression.Plant molecular biology · 2010Article
- Maize black Mexican sweet suspension cultured cells are a convenient tool for studying aquaporin activity and regulation.Plant signaling & behavior · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The osmotic water permeability coefficient (P(f)) of plasma membrane of maize (Zea mays) Black Mexican Sweet protoplasts changed dynamically during a hypoosmotic challenge, as revealed using a model-based computational approach. The best-fitting model had three free parameters: initial P(f), P(f) rate-of-change (slope(P(f))), and a delay, which were hypothesized to reflect changes in the number and/or activity of aquaporins in the plasma membrane. Remarkably, the swelling response was delayed 2 to 11 s after start of the noninstantaneous (but accounted for) bath flush. The P(f) during the delay was < or =1 microm s(-1). During the swelling period following the delay, P(f) changed dynamically: within the first 15 s P(f) either (1) increased gradually to approximately 8 microm s(-1) (in the majority population of low-initial-P(f) cells) or (2) increased abruptly to 10 to 20 microm s(-1) and then decreased gradually to 3 to 6 microm s(-1) (in the minority population of high-initial-P(f) cells). We affirmed the validity of our computational approach by the ability to reproduce previously reported initial P(f) values (including the absence of delay) in control experiments on Xenopus oocytes expressing the maize aquaporin ZmPIP2;5. Although mercury did not affect the P(f) in swelling Black Mexican Sweet cells, phloretin, another aquaporin inhibitor, inhibited swelling in a predicted manner, prolonging the delay and slowing P(f) increase, thereby confirming the hypothesis that P(f) dynamics, delay included, reflected the varying activity of aquaporins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.