ArticleBMC genomics2004
Selection and validation of endogenous reference genes using a high throughput approach.
Article in BMC genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines.Scientific reports · 2021Article
- Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica).Scientific reports · 2021Article
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- Identification of reliable reference genes for qRT-PCR studies of the developing mouse mammary gland.Scientific reports · 2016Article
- Do housekeeping genes exist?PloS one · 2015Article
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- Reference genes in real-time PCR.Journal of applied genetics · 2013Review
- Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients.Journal of translational medicine · 2013Article
- Selection of Reliable Reference Genes for Real-time qRT-PCR Analysis of Zi Geese (Anser anser domestica) Gene Expression.Asian-Australasian journal of animal sciences · 2013Article
- Cross-platform microarray meta-analysis for the mouse jejunum selects novel reference genes with highly uniform levels of expression.PloS one · 2013Article
- Correlates between host and viral transcriptional program associated with different oncolytic vaccinia virus isolates.Human gene therapy methods · 2012Article
- Regression of melanoma metastases after immunotherapy is associated with activation of antigen presentation and interferon-mediated rejection genes.International journal of cancer · 2012Article
- The stable traits of melanoma genetics: an alternate approach to target discovery.BMC genomics · 2012Article
- A signature of immune function genes associated with recurrence-free survival in breast cancer patients.Breast cancer research and treatment · 2012Article
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- Distinct signatures of the immune responses in low risk versus high risk neuroblastoma.Journal of translational medicine · 2011Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundEndogenous reference genes are commonly used to normalize expression levels of other genes with the assumption that the expression of the former is constant in different tissues and in different physiopathological conditions. Whether this assumption is correct it is, however, still matter of debate. In this study, we searched for stably expressed genes in 384 cDNA array hybridization experiments encompassing different tissues and cell lines.
resultsSeveral genes were identified whose expression was highly stable across all samples studied. The usefulness of 8 genes among them was tested by normalizing the relative gene expression against test genes whose expression pattern was known. The range of accuracy of individual endogenous reference genes was wide whereas consistent information could be obtained when information pooled from different endogenous reference genes was used.
conclusionsThis study suggests that even when the most stably expressed genes in array experiments are used as endogenous reference, significant variation in test gene expression estimates may occur and the best normalization is achieved when data from several endogenous reference genes are pooled together to minimize minimal but significant variation among samples. We are presently optimizing strategies for the preparation of endogenous reference gene mixtures that could yield information comparable to that of data pooled from individual endogenous reference gene normalizations.
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