ArticleProceedings of the National Academy of Sciences of the United States of America1992
Purification and characterization of a mammalian myosin I.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 89 citations in OpenAlex.
- Myosin isoform expressed in metastatic prostate cancer stimulates cell invasion.Scientific reports · 2017Article
- Tissue specific expression of myosin IC isoforms.BMC cell biology · 2014Article
- Article
- The myosin motor Myo1c is required for VEGFR2 delivery to the cell surface and for angiogenic signaling.American journal of physiology. Heart and circulatory physiology · 2013Article
- Myosin I and adaptation of mechanical transduction by the inner ear.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides.BMC biochemistry · 2002Article
- Myosin VIIA is specifically associated with calmodulin and microtubule-associated protein-2B (MAP-2B).The Biochemical journal · 2001Article
- Localization of myosin-Ibeta near both ends of tip links in frog saccular hair cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Myosin Ibeta is located at tip link anchors in vestibular hair bundles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- ATPase activity of myosin in hair bundles of the bullfrog's sacculus.Biophysical journal · 1997Article
- Brush border myosin-I truncated in the motor domain impairs the distribution and the function of endocytic compartments in an hepatoma cell line.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Domain structure of a mammalian myosin I beta.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Rat myr 4 defines a novel subclass of myosin I: identification, distribution, localization, and mapping of calmodulin-binding sites with differential calcium sensitivity.The Journal of cell biology · 1994Article
- The structure and function of unconventional myosins: a review.Journal of muscle research and cell motility · 1994Review
- Intracellular calcium and cAMP regulate directional pigment movements in teleost erythrophores.The Journal of cell biology · 1994Article
- Regulation of actomyosin and contraction in smooth muscle.World journal of urology · 1994Review
- Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry.The Journal of cell biology · 1993Article
- Mammalian myosin I alpha, I beta, and I gamma: new widely expressed genes of the myosin I family.The Journal of cell biology · 1993Article
- Relative distribution of actin, myosin I, and myosin II during the wound healing response of fibroblasts.The Journal of cell biology · 1993Article
- Identification, characterization and cloning of myr 1, a mammalian myosin-I.The Journal of cell biology · 1993Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction of tissue with ATP at low ionic strength and coprecipitation with actin, followed by gel filtration on Sepharose 4B, anion-exchange chromatography on Q Sepharose, and affinity chromatography on ATP-agarose. Mammalian myosin I molecules are composed of a heavy chain of 116 kDa and multiple low molecular weight polypeptides identified as calmodulin. The structural and enzymatic properties of adrenal medulla myosin I were further characterized. This enzyme exhibits high K+,EDTA- and Ca(2+)-ATPase specific activities (about 0.2 mumol.min-1 per mg of protein), whereas the Mg(2+)-ATPase activity is very low (1-3 nmol.min-1.mg-1). The Mg(2+)-ATPase of medulla myosin I is activated by F-actin in a Ca(2+)-dependent manner: activity is stimulated 40-fold in the presence of EGTA and 90-fold in the presence of 10 microM Ca2+. Two structural domains of the myosin I heavy chain were identified. A 74-kDa chymotryptic fragment contains the catalytic site, while a 36-kDa polypeptide contains the calmodulin-binding sites. These results indicate that mammalian myosin I is more closely related to myosin I from the avian intestinal brush border than to the enzymes isolated from the protozoans Acanthamoeba and Dictyostelium.
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