ArticleWorld journal of gastroenterology2004
Protective effect of melatonin against liver injury in mice induced by Bacillus Calmette-Guerin plus lipopolysaccharide.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Impact of Treatment with Antioxidants as an Adjuvant to Standard Therapy in Patients with Septic Shock: Analysis of the Correlation between Cytokine Storm and Oxidative Stress and Therapeutic Effects.International journal of molecular sciences · 2023Trial
- Usefulness of Antioxidants as Adjuvant Therapy for Septic Shock: A Randomized Clinical Trial.Medicina (Kaunas, Lithuania) · 2020Trial
- Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles.European journal of medical research · 2024Article
- Akkermansia muciniphila MucT attenuates sodium valproate-induced hepatotoxicity and upregulation of Akkermansia muciniphila in rats.Journal of cellular and molecular medicine · 2024Article
- Therapeutic potential of melatonin and melatonergic drugs on K18-hACE2 mice infected with SARS-CoV-2.Journal of pineal research · 2022Article
- Article
- Melatonin for the treatment of sepsis: the scientific rationale.Journal of thoracic disease · 2020Review
- The hepatoprotective activities ofFood science & nutrition · 2019Article
- Utilizing melatonin to combat bacterial infections and septic injury.British journal of pharmacology · 2017Review
- Effects of Melatonin on Liver Injuries and Diseases.International journal of molecular sciences · 2017Review
- Melatonin in the management of perinatal hypoxic-ischemic encephalopathy: light at the end of the tunnel?Neuropsychiatric disease and treatment · 2016Article
- Specific inhibition of histone deacetylase 8 reduces gene expression and production of proinflammatory cytokines in vitro and in vivo.The Journal of biological chemistry · 2015Article
- Immune stimulation by exogenous melatonin during experimental endotoxemia.Inflammation · 2014Article
- Neuroprotective effect of melatonin: a novel therapy against perinatal hypoxia-ischemia.International journal of molecular sciences · 2013Review
- Interaction of porcine circovirus type 2 replication with intracellular redox status in vitro.Redox report : communications in free radical research · 2013Article
- Melatonin levels in serum and ascitic fluid of patients with hepatic encephalopathy.Gastroenterology research and practice · 2012Article
- The effect of light on critical illness.Critical care (London, England) · 2011Review
- Antioxidant and preventive effects of extract from nymphaea Candida flower on in vitro immunological liver injury of rat primary hepatocyte cultures.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Hepatoprotective actions of melatonin: possible mediation by melatonin receptors.World journal of gastroenterology · 2010Review
- Role of melatonin in metabolic regulation.Reviews in endocrine & metabolic disorders · 2009Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effects and mechanisms of melatonin on immunological liver injury in mice.
methodsA model of liver injury was induced by tail vein injection of Bacillus Calmette Guerin (BCG) and lipopolysaccharide (LPS) in mice. Kupffer cells and hepatocytes were isolated and cultured according to a modified two-step collagenase perfusion technique. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and nitric oxide (NO), content of malondiadehyde (MDA), activity of superoxide dismutase (SOD), were measured by biochemical methods. Tumor necrosis factor-alpha (TNF-alpha) activity was determined by RIA. Interleukin (IL)-1 activity was measured by thymocyte proliferation bioassay. Hepatic tissue sections were stained with hematoxylin and eosin and examined under a light microscope.
resultsImmunological liver injury induced by BCG+LPS was successfully duplicated. Serum transaminase (ALT, AST) activities were significantly decreased by melatonin (0.25, 1.0, 4.0 mg/kg bm). Meanwhile, MDA content was decreased and SOD in liver homogenates was upregulated. Furthermore, pro-inflammatory mediators (TNF-alpha, IL-1, NO) in serum and liver homogenates were significantly reduced by melatonin. Histological examination demonstrated that melatonin could attenuate the area and extent of necrosis, reduce the immigration of inflammatory cells. In in vitro experiment, TNF-alpha was inhibited at the concentrations of 10(-8)-10(-6) mol/L of melatonin, while IL-1 production of Kupffer cells induced by LPS (5 microg/mL) was decreased only at the concentration of 10(-6) mol/L of melatonin, but no effect on NO production was observed. Immunological liver injury model in vitro was established by incubating hepatocytes with BCG- and LPS-induced Kupffer cells. Activities of ALT, TNF-alpha, IL-1, and MDA in supernatant were significantly increased. Melatonin had little effect on the level of ALT, but reduced the content of TNF-alpha and MDA at concentrations of 10(-7)-10(-5) mol/L and decreased the content of IL-1 at concentrations of 10(-6)-10(-5) mol/L.
conclusionMelatonin could significantly protect liver injury in mice, which was related to free radical scavenging, increased SOD activity and pro-inflammatory mediators.
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