ArticleJournal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology2004
Pharmacology of the neuronal nicotinic acetylcholine receptor of cultured Kenyon cells of the honeybee, Apis mellifera.
Article in Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 49 citations in OpenAlex.
- Pirenzepine Binding Sites in the Brain of the HoneybeeInsects · 2022Article
- The Power ofFrontiers in physiology · 2021Review
- Atypical Acetylcholine Receptors on the Neurons of the Turkish Snail.Doklady. Biochemistry and biophysics · 2020Article
- Acetylcholine and Its Receptors in Honeybees: Involvement in Development and Impairments by Neonicotinoids.Insects · 2019Review
- The Neurophysiological Bases of the Impact of Neonicotinoid Pesticides on the Behaviour of Honeybees.Insects · 2019Review
- Weight of evidence evaluation of a network of adverse outcome pathways linking activation of the nicotinic acetylcholine receptor in honey bees to colony death.The Science of the total environment · 2017Review
- Quinuclidine compounds differently act as agonists of Kenyon cell nicotinic acetylcholine receptors and induced distinct effect on insect ganglionic depolarizations.Invertebrate neuroscience : IN · 2013Article
- Cholinergic pesticides cause mushroom body neuronal inactivation in honeybees.Nature communications · 2013Article
- Pest insect olfaction in an insecticide-contaminated environment: info-disruption or hormesis effect.Frontiers in physiology · 2012Article
- An ionotropic GABA receptor in cultured mushroom body Kenyon cells of the honeybee and its modulation by intracellular calcium.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2008Article
- Study of nicotinic acetylcholine receptors on cultured antennal lobe neurones from adult honeybee brains.Invertebrate neuroscience : IN · 2008Article
- The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.Genome research · 2006Article
- Insect neuronal cultures: an experimental vehicle for studies of physiology, pharmacology and cell interactions.Invertebrate neuroscience : IN · 2006Review
- beta2-Subunit-containing nicotinic acetylcholine receptors are involved in nicotine-induced increases in conditioned reinforcement but not progressive ratio responding for food in C57BL/6 mice.Psychopharmacology · 2006Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We investigated the pharmacology of the nicotinic acetylcholine receptor of honeybee Kenyon cells, a subset of olfactory interneurons, which are crucial for olfactory learning and memory. Whole-cell currents were recorded using patch-clamp techniques. Pressure application of agonists induced inward currents in cultured Kenyon cells at holding potentials of -110 mV. Acetylcholine or carbamylcholine were full agonists, nicotine, epibatidine and cytisine were only partial agonists. Coapplications of these partial agonists with acetylcholine reduced the current amplitude. The most efficient antagonists were dihydroxy-beta-erythroidine (EC(50)=0.5 pmol x l(-1)) and methyllycaconitine (EC(50)=24 pmol x l(-1)). The open channel blocker mecamylamine, d-tubocurarine and hexamethonium were rather weak blockers of the honeybee nicotinic response. Bath applications of the muscarinic antagonist atropine inhibited nicotinic currents dependent on concentration (EC(50)=24.3 micromol x l(-1)). Muscarine, pilocarpine or oxotremorine (1 mmol x l(-1)) did not induce any measurable currents. The non-cholinergic drugs strychnine, bicuculline and picrotoxin partially and reversibly blocked the acetylcholine-induced currents. Our results indicate the expression of only one nicotinic acetylcholine receptor subtype in cultured Kenyon cells. Muscarinic as well as non-cholinergic antagonists also inhibit the receptor function, distinguishing the honeybee nicotinic receptor from the "typical" nicotinic receptor of vertebrates and from many described insects receptors.
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