ArticleProceedings of the National Academy of Sciences of the United States of America2004
Functional immunomics: microarray analysis of IgG autoantibody repertoires predicts the future response of mice to induced diabetes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 157 citations in OpenAlex.
- Oral administration of OKT3 monoclonal antibody to human subjects induces a dose-dependent immunologic effect in T cells and dendritic cells.Journal of clinical immunology · 2010Trial
- Antibody Polyreactivity: A Challenger of Immune Paradigms.Immunology · 2025Review
- Improvement of Gastrointestinal, Metabolic, and Thyroid Biomarkers through Advanced Diagnostic Techniques and Functional Nutrition Approach in 40-Year-Old Female: A Case Report.Integrative medicine (Encinitas, Calif.) · 2025Article
- Therapeutic induction of antigen-specific immune tolerance.Nature reviews. Immunology · 2024Review
- A peptide derived from HSP60 reduces proinflammatory cytokines and soluble mediators: a therapeutic approach to inflammation.Frontiers in immunology · 2023Review
- Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases.Frontiers in immunology · 2021Review
- T Follicular Regulatory Cell-Derived Fibrinogen-like Protein 2 Regulates Production of Autoantibodies and Induction of Systemic Autoimmunity.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Tolerogenic nanoparticles suppress central nervous system inflammation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries.Frontiers in immunology · 2019Article
- Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF.Journal of immunology research · 2019Article
- Human neonatal thymectomy induces altered B-cell responses and autoreactivity.European journal of immunology · 2017Article
- Antibody Epitopes Identified in Critical Regions of Dengue Virus Nonstructural 1 Protein in Mouse Vaccination and Natural Human Infections.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Antibody repertoire profiling with mimotope arrays.Human vaccines & immunotherapeutics · 2017Article
- Serum lipid antibodies are associated with cerebral tissue damage in multiple sclerosis.Neurology(R) neuroimmunology & neuroinflammation · 2016Article
- Tumor-associated and disease-associated autoantibody repertoires in healthy colostrum and maternal and newborn cord sera.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Role and therapeutic value of dendritic cells in central nervous system autoimmunity.Cell death and differentiation · 2015Review
- Article
- Pemphigus vulgaris is characterized by low IgG reactivities to specific self-antigens along with high IgG reactivity to desmoglein 3.Immunology · 2014Article
- Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays.BMC immunology · 2014Article
- Correlated matrix-assisted laser desorption/ionization mass spectrometry and fluorescent imaging of photocleavable peptide-coded random bead-arrays.Rapid communications in mass spectrometry : RCM · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One's present repertoire of antibodies encodes the history of one's past immunological experience. Can the present autoantibody repertoire be consulted to predict resistance or susceptibility to the future development of an autoimmune disease? Here, we developed an antigen microarray chip and used bioinformatic analysis to study a model of type 1 diabetes developing in nonobese diabetic male mice in which the disease was accelerated and synchronized by exposing the mice to cyclophosphamide at 4 weeks of age. We obtained sera from 19 individual mice, treated the mice to induce cyclophosphamide-accelerated diabetes (CAD), and found, as expected, that 9 mice became severely diabetic, whereas 10 mice permanently resisted diabetes. We again obtained serum from each mouse after CAD induction. We then analyzed, by using rank-order and superparamagnetic clustering, the patterns of antibodies in individual mice to 266 different antigens spotted on the chip. A selected panel of 27 different antigens (10% of the array) revealed a pattern of IgG antibody reactivity in the pre-CAD sera that discriminated between the mice resistant or susceptible to CAD with 100% sensitivity and 82% specificity (P = 0.017). Surprisingly, the set of IgG antibodies that was informative before CAD induction did not separate the resistant and susceptible groups after the onset of CAD; new antigens became critical for post-CAD repertoire discrimination. Thus, at least for a model disease, present antibody repertoires can predict future disease, predictive and diagnostic repertoires can differ, and decisive information about immune system behavior can be mined by bioinformatic technology. Repertoires matter.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.