ArticleThe Plant cell2004
The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 238 citations in OpenAlex.
- Identification of a candidate self-incompatibility locus in beet (Beta vulgaris).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- The structure and allelic diversity of the self-incompatibility locus (S-locus) in diploid potatoes inferred from genome sequences and transcriptome data from styles and pollen.The plant genome · 2025Article
- The "WHY" behind pear pollen tube growth.Plant physiology · 2025Article
- Pan-S-locus analysis reveals insights into the origin and evolution of self-incompatibility in the orange subfamily.Genome biology · 2025Article
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- PSIA: A Comprehensive Knowledgebase of Plant Self-incompatibility.Genomics, proteomics & bioinformatics · 2025Article
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- The Identification and Analysis of the Self-Incompatibility Pollen Determinant FactorPlants (Basel, Switzerland) · 2024Article
- Molecular insights into self-incompatibility systems: From evolution to breeding.Plant communications · 2024Review
- Specific functions of single pistil S-RNases in S-gene homozygous Pyrus germplasm.BMC plant biology · 2023Article
- Self-incompatibility: a targeted, unexplored pre-fertilization barrier in flower crops of Asteraceae.Journal of plant research · 2023Review
- The Pitaya Flower Tissue's Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation.International journal of molecular sciences · 2023Article
- The Snapdragon Genomes Reveal the Evolutionary Dynamics of the S-Locus Supergene.Molecular biology and evolution · 2023Article
- From the archives: Work on the mitotic spindle, root hair cell fate, and self-incompatibility highlights the wonderful world of the plant cell.The Plant cell · 2022Article
- Origin, loss, and regain of self-incompatibility in angiosperms.The Plant cell · 2022Article
- Primary restriction of S-RNase cytotoxicity by a stepwise ubiquitination and degradation pathway in Petunia hybrida.The New phytologist · 2021Article
- The identification of the Rosa S-locus and implications on the evolution of the Rosaceae gametophytic self-incompatibility systems.Scientific reports · 2021Article
- Article
- Genome structure and evolution of Antirrhinum majus L.Nature plants · 2019Article
- Finding a Compatible Partner: Self-Incompatibility in European Pear (Frontiers in plant science · 2019Review
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S(2) plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone (TAC26) containing both AhSLF-S(2) and AhS(2)-RNase into a self-incompatible (SI) line of Petunia hybrida. Molecular analyses showed that both genes are correctly expressed in pollen and pistil in four independent transgenic lines of petunia. Pollination tests indicated that all four lines became self-compatible because of the specific loss of the pollen function of SI. This alteration was transmitted stably into the T1 progeny. We then transformed AhSLF-S(2) cDNA under the control of a tomato (Lycopersicon esculentum) pollen-specific promoter LAT52 into the self-incompatible petunia line. Molecular studies revealed that AhSLF-S(2) is specifically expressed in pollen of five independent transgenic plants. Pollination tests showed that they also had lost the pollen function of SI. Importantly, expression of endogenous SLF or SLF-like genes was not altered in these transgenic plants. These results phenocopy a well-known phenomenon called competitive interaction whereby the presence of two different pollen S alleles within pollen leads to the breakdown of the pollen function of SI in several solanaceaous species. Furthermore, we demonstrated that AhSLF-S(2) physically interacts with PhS(3)-RNase from the P. hybrida line used for transformation. Together with the recent demonstration of PiSLF as the pollen determinant in P. inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S(2) controls the pollen function of S-RNase-based self-incompatibility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.