ArticleJournal of virology2004
Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
- Discovering the complete enhancer map of human herpesviruses using a natural language processing model.Nature communications · 2025Article
- RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production.PloS one · 2021Article
- Comparative Analysis of Gammaherpesvirus Circular RNA Repertoires: Conserved and Unique Viral Circular RNAs.Journal of virology · 2019Article
- RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency.Pathogens (Basel, Switzerland) · 2017Article
- Multiple Lytic Origins of Replication Are Required for Optimal Gammaherpesvirus Fitness In Vitro and In Vivo.PLoS pathogens · 2016Article
- Human cytomegalovirus pUL79 is an elongation factor of RNA polymerase II for viral gene transcription.PLoS pathogens · 2014Article
- Unconventional sequence requirement for viral late gene core promoters of murine gammaherpesvirus 68.Journal of virology · 2014Article
- Murine cytomegalovirus protein pM92 is a conserved regulator of viral late gene expression.Journal of virology · 2014Article
- CTCF and Sp1 interact with the Murine gammaherpesvirus 68 internal repeat elements.Virus genes · 2012Article
- The Epstein-Barr virus BcRF1 gene product is a TBP-like protein with an essential role in late gene expression.Journal of virology · 2012Article
- Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells.Virology · 2011Article
- Replication and transcription activator (RTA) of murine gammaherpesvirus 68 binds to an RTA-responsive element and activates the expression of ORF18.Journal of virology · 2011Article
- CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection.Protein & cell · 2011Article
- The human cytomegalovirus gene UL79 is required for the accumulation of late viral transcripts.Journal of virology · 2011Article
- Least-Squares Support Vector Machine Approach to Viral Replication Origin Prediction.INFORMS journal on computing · 2010Article
- Initiation of lytic DNA replication in Epstein-Barr virus: search for a common family mechanism.Future virology · 2010Article
- The M10 locus of murine gammaherpesvirus 68 contributes to both the lytic and the latent phases of infection.Journal of virology · 2009Article
- Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68.Virology · 2009Article
- ORF30 and ORF34 are essential for expression of late genes in murine gammaherpesvirus 68.Journal of virology · 2009Article
- A repetitive region of gammaherpesvirus genomic DNA is a ligand for induction of type I interferon.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human gammaherpesviruses are associated with lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) infection of mice has emerged as a model for understanding gammaherpesvirus pathogenesis in vivo. In contrast to human gammaherpesviruses, MHV-68 replicates in permissive cell lines in a robust manner, presenting an efficient model to study the basic mechanisms for DNA replication and recombination processes. In addition, MHV-68 also infects a broad range of cells of different tissue types and from different host species, and the viral genome persists as an episome in infected cells. These features make MHV-68 an attractive system on which to build gene delivery vectors. We have therefore undertaken a study to identify the cis elements required for MHV-68 genome replication and packaging. Here we report that an 8.4-kb MHV-68 genomic fragment between ORF66 and ORF73 conferred on the plasmid the ability to replicate; replication required the presence of either de novo viral infection or viral reactivation from latency. We further mapped the origin of lytic replication (oriLyt) to a 1.25-kb region. Moreover, we demonstrated that the terminal repeat of the viral genome is sufficient for packaging of the replicated oriLyt plasmid into mature viral particles. Functional identification of the MHV-68 oriLyt and packaging signal has laid a foundation for investigating the mechanisms controlling gammaherpesvirus DNA replication during the viral lytic phase and will also serve as a base on which to design gene delivery vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.