ArticleJournal of virology2004
The N-terminal region of the murine coronavirus spike glycoprotein is associated with the extended host range of viruses from persistently infected murine cells.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- The Role of Host Genetic Factors in Coronavirus Susceptibility: Review of Animal and Systematic Review of Human Literature.American journal of human genetics · 2020Pooled it
- Three Amino Acid Substitutions in the Spike Protein Enable the Coronavirus Porcine Epidemic Diarrhea Virus To Infect Vero Cells.Microbiology spectrum · 2023Article
- Review
- Article
- Review
- Natural and Recombinant SARS-CoV-2 Isolates Rapidly EvolveJournal of virology · 2021Article
- Molecular diversity of coronavirus host cell entry receptors.FEMS microbiology reviews · 2021Review
- There is no evidence of SARS-CoV-2 laboratory origin: Response to Segreto and Deigin (DOI: 10.1002/bies.202000240).BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Article
- SARS-CoV-2 and COVID-19: A genetic, epidemiological, and evolutionary perspective.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2020Review
- Coding potential and sequence conservation of SARS-CoV-2 and related animal viruses.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2020Article
- Computational Inference of Selection Underlying the Evolution of the Novel Coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2.Journal of virology · 2020Article
- First detection of bovine coronavirus in Yak (Bos grunniens) and a bovine coronavirus genome with a recombinant HE gene.The Journal of general virology · 2019Article
- Geographical structure of bat SARS-related coronaviruses.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2019Review
- Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China.Veterinary microbiology · 2017Article
- Contributions of the S2 spike ectodomain to attachment and host range of infectious bronchitis virus.Virus research · 2013Article
- Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission.Journal of virology · 2010Review
- Non-invasive imaging of mouse hepatitis coronavirus infection reveals determinants of viral replication and spread in vivo.Cellular microbiology · 2009Article
- Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cleavage of group 1 coronavirus spike proteins: how furin cleavage is traded off against heparan sulfate binding upon cell culture adaptation.Journal of virology · 2008Article
- Amino acid substitutions in the S2 subunit of mouse hepatitis virus variant V51 encode determinants of host range expansion.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Although murine coronaviruses naturally infect only mice, several virus variants derived from persistently infected murine cell cultures have an extended host range. The mouse hepatitis virus (MHV) variant MHV/BHK can infect hamster, rat, cat, dog, monkey, and human cell lines but not the swine testis (ST) porcine cell line (J. H. Schickli, B. D. Zelus, D. E. Wentworth, S. G. Sawicki, and K. V. Holmes, J. Virol. 71:9499-9507, 1997). The spike (S) gene of MHV/BHK had 63 point mutations and a 21-bp insert that encoded 56 amino acid substitutions and a 7-amino-acid insert compared to the parental MHV strain A59. Recombinant viruses between MHV-A59 and MHV/BHK were selected in hamster cells. All of the recombinants retained 21 amino acid substitutions and a 7-amino-acid insert found in the N-terminal region of S of MHV/BHK, suggesting that these residues were responsible for the extended host range of MHV/BHK. Flow cytometry showed that MHV-A59 bound only to cells that expressed the murine glycoprotein receptor CEACAM1a. In contrast, MHV/BHK and a recombinant virus, k6c, with the 21 amino acid substitutions and 7-amino-acid insert in S bound to hamster (BHK) and ST cells as well as murine cells. Thus, 21 amino acid substitutions and a 7-amino-acid insert in the N-terminal region of the S glycoprotein of MHV/BHK confer the ability to bind and in some cases infect cells of nonmurine species.
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Registered trials
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