ArticleThe Journal of physiology2004
Regulation of human airway ciliary beat frequency by intracellular pH.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 78 citations in OpenAlex.
- T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection.The Journal of clinical investigation · 2012Trial
- Human Nasal and Bronchial Epithelium in Cystic Fibrosis: Differences in Expression of Airway pH Regulatory Proteins.Pediatric pulmonology · 2026Article
- Article
- Assessment of liquid media requirements for storing and evaluating respiratory cilia motility.PeerJ · 2025Article
- The effect of maxillary sinus floor elevation with lateral antrostomy approach on nasal mucociliary functions.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2025Article
- Ciliary Motility Decreased by a COInternational journal of molecular sciences · 2024Article
- The Increase in the Frequency and Amplitude of the Beating of Isolated Mouse Tracheal Cilia Reactivated by ATP and cAMP with Elevation in pH.International journal of molecular sciences · 2024Article
- Dopamine, norepinephrine, and vasopressin accelerate particle transport velocity in murine tracheal epithelium via substance-specific receptor pathways: dependency on intra- and extracellular CaFrontiers in pharmacology · 2024Article
- Ambroxol-Enhanced Frequency and Amplitude of Beating Cilia Controlled by a Voltage-Gated CaInternational journal of molecular sciences · 2023Article
- Effects of condensates from volcanic fumaroles and cigarette smoke extracts on airway epithelial cells.Human cell · 2023Article
- A 20:1 synergetic mixture of cafedrine/theodrenaline accelerates particle transport velocity in murine tracheal epithelium via IPFrontiers in pharmacology · 2023Article
- Enhancement of airway ciliary beating mediated via voltage-gated CaPflugers Archiv : European journal of physiology · 2022Article
- Echinocandins Accelerate Particle Transport Velocity in the Murine Tracheal Epithelium: Dependency on Intracellular CaAntimicrobial agents and chemotherapy · 2021Article
- Proteases, Mucus, and Mucosal Immunity in Chronic Lung Disease.International journal of molecular sciences · 2021Review
- Intracellular ClInternational journal of molecular sciences · 2020Review
- Acid exposure disrupts mucus secretion and impairs mucociliary transport in neonatal piglet airways.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps.Frontiers in cell and developmental biology · 2020Article
- Ciliary beating amplitude controlled by intracellular ClPflugers Archiv : European journal of physiology · 2019Article
- Carbocisteine stimulated an increase in ciliary bend angle via a decrease in [ClPflugers Archiv : European journal of physiology · 2019Article
- Construction of a Suite of Computable Biological Network Models Focused on Mucociliary Clearance in the Respiratory Tract.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
pHi affects a number of cellular functions, but the influence of pHi on mammalian ciliary beat frequency (CBF) is not known. CBF and pHi of single human tracheobronchial epithelial cells in submerged culture were measured simultaneously using video microscopy (for CBF) and epifluorescence microscopy with the pH-sensitive dye BCECF. Baseline CBF and pHi values in bicarbonate-free medium were 7.2 +/- 0.2 Hz and 7.49 +/- 0.02, respectively (n = 63). Alkalization by ammonium pre-pulse to pHi 7.78 +/- 0.02 resulted in a 2.2 +/- 0.1 Hz CBF increase (P < 0.05). Following removal of NH4Cl, pHi decreased to 7.24 +/- 0.02 and CBF to 5.8 +/- 0.1 Hz (P < 0.05). Removal of extracellular CO2 to change pHi resulted in similar CBF changes. Pre-activation of cAMP-dependent protein kinase (10 microM forskolin), broad inhibition of protein kinases (100 microM H-7), inhibition of PKA (10 microM H-89), nor inhibition of phosphatases (10 microM cyclosporin + 1.5 microM okadaic acid) changed pHi-mediated changes in CBF, nor were they due to [Ca2+]i changes. CBF of basolaterally permeabilized human tracheobronchial cells, re-differentiated at the air-liquid interface, was 3.9 +/- 0.3, 5.7 +/- 0.4, 7.0 +/- 0.3 and 7.3 +/- 0.3 Hz at basolateral i.e., intracellular pH of 6.8, 7.2, 7.6 and 8.0, respectively (n = 18). Thus, intracellular alkalization stimulates, while intracellular acidification attenuates human airway CBF. Since phosphorylation and [Ca2+]i changes did not seem to mediate pHi-induced CBF changes, pHi may directly act on the ciliary motile machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.