ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Ecto-nucleotidases and nucleoside transporters mediate activation of adenosine receptors on hippocampal mossy fibers by P2X7 receptor agonist 2'-3'-O-(4-benzoylbenzoyl)-ATP.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 79 citations in OpenAlex.
- Genetic Deletion of Adenosine AGlia · 2026Article
- Purinergic agonists increase [CaAmerican journal of physiology. Cell physiology · 2024Article
- P2X7 purinergic receptor modulates dentate gyrus excitatory neurotransmission and alleviates schizophrenia-like symptoms in mouse.iScience · 2023Article
- Crosstalk Between ATP-PFrontiers in cellular neuroscience · 2021Article
- P2X7 Receptor Agonist 2'(3')-O-(4-Benzoylbenzoyl)ATP Differently Modulates Cell Viability and Corticostriatal Synaptic Transmission in Experimental Models of Huntington's Disease.Frontiers in pharmacology · 2020Article
- Inhibition of vascular smooth muscle cell calcification by ATP analogues.Purinergic signalling · 2019Article
- P2X7 Receptor Signaling in Stress and Depression.International journal of molecular sciences · 2019Review
- Extracellular ATP and IL-23 Form a Local Inflammatory Circuit Leading to the Development of a Neutrophil-Dependent Psoriasiform Dermatitis.The Journal of investigative dermatology · 2018Article
- Modulation of Glucose Takeup by Glucose Transport on the Isolated OHCs.Neural plasticity · 2018Article
- Neuronal P2X7 Receptors Revisited: Do They Really Exist?The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- Neuronal P2X7 Receptor: Involvement in Neuronal Physiology and Pathology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
- P2XPurinergic signalling · 2016Article
- Microglia-derived purines modulate mossy fibre synaptic transmission and plasticity through P2X4 and A1 receptors.The European journal of neuroscience · 2016Article
- Purinergic responses of calcium-dependent signaling pathways in cultured adult human astrocytes.BMC neuroscience · 2014Article
- P2X receptors in neuroglia.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Entorhinal theta-frequency input to the dentate gyrus trisynaptically evokes hippocampal CA1 LTP.Frontiers in neural circuits · 2012Article
- Stimulation of the P2X7 receptor kills rat retinal ganglion cells in vivo.Experimental eye research · 2010Article
- P2 receptors in renal pathophysiology.Purinergic signalling · 2009Article
- Activation of adenosine A2B receptors enhances ciliary beat frequency in mouse lateral ventricle ependymal cells.Cerebrospinal fluid research · 2009Article
- Physiological and pathological functions of P2X7 receptor in the spinal cord.Purinergic signalling · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ionotropic and cytolytic P2X7 receptor is typically found on immune cells, where it is involved in the release of cytokines. Recently, P2X7 receptors were reported to be localized to presynaptic nerve terminals and to modulate transmitter release. In the present study, we reassessed this unexpected role of P2X7 receptors at hippocampal mossy fiber-CA3 synapses. In agreement with previous findings, the widely used P2X7 agonist 2'-3'-O-(4-benzoylbenzoyl)-adenosine-5'-triphosphate (BzATP) clearly depressed field potentials (fEPSPs); however, no evidence for an involvement of P2X7 receptors could be obtained. First, depression of fEPSPs by BzATP was unchanged in P2X7-/- mice. Second, experiments using P2X7-/- mice, immunohistochemistry, and electron microscopy showed that the antigen detected by frequently used P2X7 antibodies is not compatible with a plasmalemmal P2X7 receptor. Third, BzATP did not alter Ca2+ levels in synaptic terminals. In contrast, the depression of fEPSPs by BzATP was fully blocked by adenosine (A1) receptor antagonists. Furthermore, the application of BzATP also activated postsynaptic A1 receptor-coupled K+ channels. This effect of BzATP was mimicked by ATP and adenosine and was completely prevented by enzymes specifically degrading adenosine. Activation of A1-coupled K+ channels by BzATP was dependent on ecto-nucleotidases, extracellular enzymes that convert ATP to adenosine. Moreover, the opening of A1-coupled K+ channels by BzATP was dependent on nucleoside transporters. Taken together, our results indicate that BzATP is extracellularly catabolized to Bz-adenosine and subsequently hetero-exchanged for intracellular adenosine and then depresses mossy fiber fEPSPs through presynaptic A1 receptors rather than through P2X7 receptors. Thus, the present study casts doubts on the neuronal localization of P2X7 receptors in rodent hippocampus.
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