ArticleProceedings of the National Academy of Sciences of the United States of America2004
Evolutionarily conserved sequence elements that positively regulate IFN-gamma expression in T cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 137 citations in OpenAlex.
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- Gene Regulatory Programs of NK Cells Show That NCAM1 (CD56) and KIRs Are Controlled by Genetically Polymorphic Distal Regulatory Elements.European journal of immunology · 2026Article
- Essential roles of CTCF binding sites at TAD boundaries in modulating chromatin interactions and transcriptional regulation at theFrontiers in immunology · 2025Article
- Regulation of T helper cell differentiation by the interplay between histone modification and chromatin interaction.Immunity · 2024Article
- Dynamic single-cell regulomes characterize human peripheral blood innate lymphoid cell subpopulations.iScience · 2023Article
- Restraint of IFN-γ expression through a distal silencer CNS-28 for tissue homeostasis.Immunity · 2023Article
- Interferon-γ: teammate or opponent in the tumour microenvironment?Nature reviews. Immunology · 2022Review
- The Th1 cell regulatory circuitry is largely conserved between human and mouse.Life science alliance · 2021Article
- Re-Programming Autoreactive T Cells Into T-Regulatory Type 1 Cells for the Treatment of Autoimmunity.Frontiers in immunology · 2021Review
- A Two-Step Process of Effector Programming Governs CD4Cell reports · 2020Article
- STAT4 Directs a Protective Innate Lymphoid Cell Response to Gastrointestinal Infection.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- A conserved enhancer regulates Il9 expression in multiple lineages.Nature communications · 2018Article
- 14-3-3z sequesters cytosolic T-bet, upregulating IL-13 levels in TThe Journal of allergy and clinical immunology · 2018Article
- Review
- JunB promotes Th17 cell identity and restrains alternative CD4Nature communications · 2017Article
- Genetic variants alter T-bet binding and gene expression in mucosal inflammatory disease.PLoS genetics · 2017Article
- Rapid Recall Ability of Memory T cells is Encoded in their Epigenome.Scientific reports · 2017Article
- Crystal structure of the DNA binding domain of the transcription factor T-bet suggests simultaneous recognition of distant genome sites.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats.International journal of molecular sciences · 2016Article
- BACH2 regulates CD8(+) T cell differentiation by controlling access of AP-1 factors to enhancers.Nature immunology · 2016Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Our understanding of mechanisms by which the expression of IFN-gamma is regulated is limited. Herein, we identify two evolutionarily conserved noncoding sequence elements (IFNgCNS1 and IFNg CNS2) located approximately 5 kb upstream and approximately 18 kb downstream of the initiation codon of the murine Ifng gene. When linked to the murine Ifng gene (-3.4 to +5.6 kb) and transiently transfected into EL-4 cells, these elements clearly enhanced IFN-gamma expression in response to ionomycin and phorbol 12-myristate 13-acetate and weakly enhanced expression in response to T-bet. A DNase I hypersensitive site and extragenic transcripts at IFNgCNS2 correlated positively with the capacity of primary T cell subsets to produce IFN-gamma. Transcriptionally favorable histone modifications in the Ifng promoter, intronic regions, IFNgCNS2, and, although less pronounced, IFNgCNS1 increased as naïve T cells differentiated into IFN-gamma-producing effector CD8+ and T helper (TH) 1 T cells, but not into TH2 T cells. Like IFN-gamma expression, these histone modifications were T-bet-dependent in CD4+ cells, but not CD8+ T cells. These findings define two distal regulatory elements associated with T cell subset-specific IFN-gamma expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.