ArticleNucleic acids research2004
Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
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- Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1.Nucleic acids research · 2019Article
- Experimental maps of DNA structure at nucleotide resolution distinguish intrinsic from protein-induced DNA deformations.Nucleic acids research · 2018Article
- Genome-wide prediction of minor-groove electrostatic potential enables biophysical modeling of protein-DNA binding.Nucleic acids research · 2017Article
- NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.Protein science : a publication of the Protein Society · 2017Article
- DNA Transposition at Work.Chemical reviews · 2016Review
- Crystallization of and selenomethionine phasing strategy for a SETMAR-DNA complex.Acta crystallographica. Section F, Structural biology communications · 2016Article
- The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications.Nucleic acids research · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Structural role of the flanking DNA in mariner transposon excision.Nucleic acids research · 2015Article
- NMR structural analysis of Sleeping Beauty transposase binding to DNA.Protein science : a publication of the Protein Society · 2014Article
- Article
- Isolation and characterization of seventy-nine full-length mariner-like transposase genes in the Bambusoideae subfamily.Journal of plant research · 2011Article
- A DNA minor groove electronegative potential genome map based on photo-chemical probing.Nucleic acids research · 2011Article
- DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans.The EMBO journal · 2010Article
- DDE transposases: Structural similarity and diversity.Advanced drug delivery reviews · 2010Review
- Review
- Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.Critical reviews in biochemistry and molecular biology · 2010Review
- The role of DNA shape in protein-DNA recognition.Nature · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bipartite DNA-binding domain of Tc3 transposase, Tc3A, was crystallized in complex with its transposon recognition sequence. In the structure the two DNA-binding domains form structurally related helix-turn-helix (HTH) motifs. They both bind to the major groove on a single DNA oligomer, separated by a linker that interacts closely with the minor groove. The structure resembles that of the transcription factor Pax6 DNA-binding domain, but the relative orientation of the HTH-domain is different. The DNA conformation is distorted, characterized by local narrowing of the minor groove and bends at both ends. The protein-DNA recognition takes place through base and backbone contacts, as well as shape-recognition of the distortions in the DNA. Charged interactions are primarily found in the N-terminal domain and the linker indicating that these may form the initial contact area. Two independent dimer interfaces could be relevant for bringing together transposon ends and for binding to a direct repeat site in the transposon end. In contrast to the Tn5 synaptic complex, the two Tc3A DNA-binding domains bind to a single Tc3 transposon end.
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