ArticleNucleic acids research2004
Induction and characterization of taxol-resistance phenotypes with a transiently expressed artificial transcriptional activator library.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Reprogramming cell fate with artificial transcription factors.FEBS letters · 2018Review
- Controlling gene networks and cell fate with precision-targeted DNA-binding proteins and small-molecule-based genome readers.The Biochemical journal · 2014Review
- Induction of stable drug resistance in human breast cancer cells using a combinatorial zinc finger transcription factor library.PloS one · 2011Article
- Modular system for the construction of zinc-finger libraries and proteins.Nature protocols · 2010Article
- Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly.Genome research · 2009Article
- Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells.Journal of bacteriology · 2005Article
- Epigenetic manipulation of gene expression: a toolkit for cell biologists.The Journal of cell biology · 2005Review
- Role of cytochrome P450 activity in the fate of anticancer agents and in drug resistance: focus on tamoxifen, paclitaxel and imatinib metabolism.Clinical pharmacokinetics · 2005Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenotype-based functional genomic methods are useful for the identification of genes that are related to a particular biological function or disease. Essential to this approach is the ability to regulate the expression of selected genes. Artificial transcription factors (ATFs) are key molecular tools that selectively regulate gene expression in vivo. Here, we use an ATF library to identify genes that participate in rendering a cell resistant to the drug Taxol, a potent anti-cancer drug that binds to tubulin and inhibits cell division. The library, which encodes ATFs that activate (rather than inhibit) transcription, was introduced into a HeLa cell line, and Taxol-resistant cells were selected. After eight rounds of selection, we identified two ATFs that significantly increased the level of Taxol resistance (TR) in HeLa cells. Gene expression microarray experiments using these ATFs identified 37 co-regulated genes, including genes already known to participate in TR. This study demonstrates that ATF libraries can be used to induce phenotypic alterations in eukaryotic cells and then identify specific genes that are associated with the phenotype of choice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.