ArticleMolecular biology of the cell2004
The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- Composition and function of ciliary inner-dynein-arm subunits studied in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- LAX28 is required for the stable assembly of the inner dynein arm f complex, and the tether and tether head complex inJournal of cell science · 2020Article
- The Carboxyl Terminus of Tegument Protein pUL21 Contributes to Pseudorabies Virus Neuroinvasion.Journal of virology · 2019Article
- Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants.Nature communications · 2018Article
- A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1).Molecular biology of the cell · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Structure and function of outer dynein arm intermediate and light chain complex.Molecular biology of the cell · 2016Article
- Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum.Nature communications · 2015Article
- The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2014Review
- CMF22 is a broadly conserved axonemal protein and is required for propulsive motility in Trypanosoma brucei.Eukaryotic cell · 2013Article
- Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein.FEBS letters · 2013Article
- Recessive HYDIN mutations cause primary ciliary dyskinesia without randomization of left-right body asymmetry.American journal of human genetics · 2012Article
- Integrated control of axonemal dynein AAA(+) motors.Journal of structural biology · 2012Review
- Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- bop5 Mutations reveal new roles for the IC138 phosphoprotein in the regulation of flagellar motility and asymmetric waveforms.Molecular biology of the cell · 2011Article
- A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii.Cell motility and the cytoskeleton · 2009Article
- IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding.Molecular biology of the cell · 2009Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Members of the LC7/Roadblock family of light chains (LCs) have been found in both cytoplasmic and axonemal dyneins. LC7a was originally identified within Chlamydomonas outer arm dynein and associates with this motor's cargo-binding region. We describe here a novel member of this protein family, termed LC7b that is also present in the Chlamydomonas flagellum. Levels of LC7b are reduced approximately 20% in axonemes isolated from strains lacking inner arm I1 and are approximately 80% lower in the absence of the outer arms. When both dyneins are missing, LC7b levels are diminished to <10%. In oda9 axonemal extracts that completely lack outer arms, LC7b copurifies with inner arm I1, whereas in ida1 extracts that are devoid of I1 inner arms it associates with outer arm dynein. We also have observed that some LC7a is present in both isolated axonemes and purified 18S dynein from oda1, suggesting that it is also a component of both the outer arm and inner arm I1. Intriguingly, in axonemal extracts from the LC7a null mutant, oda15, which assembles approximately 30% of its outer arms, LC7b fails to copurify with either dynein, suggesting that it interacts with LC7a. Furthermore, both the outer arm gamma heavy chain and DC2 from the outer arm docking complex completely dissociate after salt extraction from oda15 axonemes. EDC cross-linking of purified dynein revealed that LC7b interacts with LC3, an outer dynein arm thioredoxin; DC2, an outer arm docking complex component; and also with the phosphoprotein IC138 from inner arm I1. These data suggest that LC7a stabilizes both the outer arms and inner arm I1 and that both LC7a and LC7b are involved in multiple intradynein interactions within both dyneins.
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