ArticleBMC developmental biology2004
MPSS profiling of human embryonic stem cells.
Article in BMC developmental biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas.Stem cells (Dayton, Ohio) · 2007Pooled it
- Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells.Cells · 2021Article
- Decoding pluripotency: Genetic screens to interrogate the acquisition, maintenance, and exit of pluripotency.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Techniques and Approaches to Genetic Analyses in Nephrological Disorders.Journal of pediatric genetics · 2016Review
- Pluripotent Stem Cells: Current Understanding and Future Directions.Stem cells international · 2016Review
- Current methods and challenges in the comprehensive characterization of human pluripotent stem cells.Stem cell reviews and reports · 2015Review
- Transcriptomics and proteomics in stem cell research.Frontiers of medicine · 2014Review
- Comparative gene expression signature of pig, human and mouse induced pluripotent stem cell lines reveals insight into pig pluripotency gene networks.Stem cell reviews and reports · 2014Article
- Germ cell specification and pluripotency in mammals: a perspective from early embryogenesis.Reproductive medicine and biology · 2014Article
- Reviewing and updating the major molecular markers for stem cells.Stem cells and development · 2013Review
- Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human embryonic stem cells.Genome research · 2011Article
- Computational analysis of expression of human embryonic stem cell-associated signatures in tumors.BMC research notes · 2011Article
- Rare cell proteomic reactor applied to stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics study of human embryonic stem cell differentiation.Molecular & cellular proteomics : MCP · 2011Article
- DIA1R is an X-linked gene related to Deleted In Autism-1.PloS one · 2011Article
- Article
- Human embryonic stem cells: derivation, culture, and differentiation: a review.Restorative neurology and neuroscience · 2010Review
- Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells.Molecular cell · 2009Article
- Gene expression profiles during early differentiation of mouse embryonic stem cells.BMC developmental biology · 2009Article
- Validation of oligoarrays for quantitative exploration of the transcriptome.BMC genomics · 2008Article
- Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment.Cell stem cell · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPooled human embryonic stem cells (hESC) cell lines were profiled to obtain a comprehensive list of genes common to undifferentiated human embryonic stem cells.
resultsPooled hESC lines were profiled to obtain a comprehensive list of genes common to human ES cells. Massively parallel signature sequencing (MPSS) of approximately three million signature tags (signatures) identified close to eleven thousand unique transcripts, of which approximately 25% were uncharacterised or novel genes. Expression of previously identified ES cell markers was confirmed and multiple genes not known to be expressed by ES cells were identified by comparing with public SAGE databases, EST libraries and parallel analysis by microarray and RT-PCR. Chromosomal mapping of expressed genes failed to identify major hotspots and confirmed expression of genes that map to the X and Y chromosome. Comparison with published data sets confirmed the validity of the analysis and the depth and power of MPSS.
conclusionsOverall, our analysis provides a molecular signature of genes expressed by undifferentiated ES cells that can be used to monitor the state of ES cells isolated by different laboratories using independent methods and maintained under differing culture conditions
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.