ArticleProceedings of the National Academy of Sciences of the United States of America2004
Translation arrest of SecM is essential for the basal and regulated expression of SecA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 76 citations in OpenAlex.
- Structural insights into YheS-mediated release of SecM-arrested ribosome.Nature communications · 2026Article
- Structural basis of enhanced stalling efficiency of an engineered ribosome arrest peptide.Nucleic acids research · 2025Article
- The ABCF proteins in Escherichia coli individually cope with 'hard-to-translate' nascent peptide sequences.Nucleic acids research · 2024Article
- Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery.Nature communications · 2024Article
- Article
- The ribosome stabilizes partially folded intermediates of a nascent multi-domain protein.Nature chemistry · 2022Article
- A nascent polypeptide sequence modulates DnaA translation elongation in response to nutrient availability.eLife · 2021Article
- Translational regulation of environmental adaptation in bacteria.The Journal of biological chemistry · 2020Review
- Ddi1 is a ubiquitin-dependent protease.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural insights into the mechanism of a novel protein targeting pathway in Gram-negative bacteria.FEBS open bio · 2020Article
- Electrostatic Interactions Govern Extreme Nascent Protein Ejection Times from Ribosomes and Can Delay Ribosome Recycling.Journal of the American Chemical Society · 2020Article
- Increased expression of the bacterial glycolipid MPIase is required for efficient protein translocation across membranes in cold conditions.The Journal of biological chemistry · 2019Article
- Using the Bacterial Ribosome as a Discovery Platform for Peptide-Based Antibiotics.Biochemistry · 2019Review
- Article
- Genome scale analysis of Escherichia coli with a comprehensive prokaryotic sequence-based biophysical model of translation initiation and elongation.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Identification and characterization of a translation arrest motif in VemP by systematic mutational analysis.The Journal of biological chemistry · 2018Article
- The Essential Genome ofmBio · 2018Article
- The ribosome and its role in protein folding: looking through a magnifying glass.Acta crystallographica. Section D, Structural biology · 2017Review
- Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SecM protein of Escherichia coli contains an arrest sequence (F(150)XXXXWIXXXXGIRAGP(166)), which interacts with the ribosomal exit tunnel to halt translation elongation beyond Pro-166. This inhibition is reversed by active export of the nascent SecM chain. Here, we studied the physiological roles of SecM. Arrest-alleviating mutations in the arrest sequence reduced the expression of secA, a downstream gene on the same mRNA. Among such mutations, the arrest-abolishing P166A substitution mutation on the chromosomal secM gene proved lethal unless the mutant cells are complemented with excess SecA. Whereas secretion defect due either to azide addition, a secY mutation, or low temperature leads to up-regulated SecA biosynthesis, this regulation was lost by a secM mutation, which synergistically retarded growth of cells with lowered secretion activity. Finally, an arrest-alleviating rRNA mutation affecting the constricted part of the exit tunnel lowered the basal level of SecA as well as its secretion defect-induced up-regulation. Thus, the arrest sequence of SecM has at least two roles in SecA translation. First, the transient elongation arrest in normal cells is required for the synthesis of SecA at levels sufficient to support cell growth. Second, the prolonged SecM elongation arrest under conditions of unfavorable protein secretion is required for the enhanced expression of SecA to cope with such conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.