ArticleNucleic acids research2004
RNAi-dependent and RNAi-independent mechanisms contribute to the silencing of RIPed sequences in Neurospora crassa.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Tandem repeat-induced sexual silencing: A Rid-dependent RNAi mechanism for fungal genome defense via translational repression.Science advances · 2025Article
- Fungi as models of centromere innovation: from DNA sequence to 3-dimensional arrangement.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- H3T11 phosphorylation by CKII is required for heterochromatin formation in Neurospora.Nucleic acids research · 2024Article
- Epigenetic modulation of fungal pathogens: a focus onFrontiers in microbiology · 2024Review
- Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.Microorganisms · 2020Review
- Extracellular Vesicle-Mediated RNA Release inmSphere · 2019Article
- Epigenetic regulation of development and pathogenesis in fungal plant pathogens.Molecular plant pathology · 2017Review
- Mechanism of siRNA production from repetitive DNA.Genes & development · 2015Article
- Recognizing the enemy within: licensing RNA-guided genome defense.Trends in biochemical sciences · 2014Review
- Article
- Neurospora crassa, a model system for epigenetics research.Cold Spring Harbor perspectives in biology · 2013Review
- Article
- DIRS and Ngaro Retrotransposons in Fungi.PloS one · 2013Article
- Centromeres of filamentous fungi.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles.Genome research · 2011Article
- Heterochromatin is required for normal distribution of Neurospora crassa CenH3.Molecular and cellular biology · 2011Article
- LTR retrotransposons in fungi.PloS one · 2011Article
- Characterizing the role of RNA silencing components in Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2010Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) can silence genes at the transcriptional level by targeting locus-specific Lys9H3 methylation or at the post-transcriptional level by targeting mRNA degradation. Here we have cloned and sequenced genomic regions methylated in Lys9H3 in Neurospora crassa to test the requirements for components of the RNAi pathway in this modification. We find that 90% of clones map to repeated sequences and relics of transposons that have undergone repeat-induced point mutations (RIP). We find siRNAs derived from transposon relics indicating that the RNAi machinery targets these regions. This is confirmed by the fact that the presence of these siRNAs depends on components of the RNAi pathway such as the RdRP (QDE-1), the putative RecQ helicase (QDE-3) and the two Dicer enzymes. We show that Lys9H3 methylation of RIP sequences is not affected in mutants of the RNAi pathway indicating that the RNAi machinery is not involved in transcriptional gene silencing in Neurospora. We find that RIP regions are transcribed and that the transcript level increases in the mutants of the RNAi pathway. These data suggest that the biological function of the Neurospora RNAi machinery is to control transposon relics and repeated sequences by targeting degradation of transcripts derived from these regions.
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